PUBLICATION
Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals
- Authors
- Gawriluk, T.R., Simkin, J., Thompson, K.L., Biswas, S.K., Clare-Salzler, Z., Kimani, J.M., Kiama, S.G., Smith, J.J., Ezenwa, V.O., Seifert, A.W.
- ID
- ZDB-PUB-160426-3
- Date
- 2016
- Source
- Nature communications 7: 11164 (Journal)
- Registered Authors
- Smith, Jeramiah
- Keywords
- Cell division, Cell proliferation, Regeneration
- MeSH Terms
-
- Animals
- Cell Cycle/genetics
- Cell Cycle/physiology
- Cell Proliferation/genetics
- Cell Proliferation/physiology
- Ear, External/injuries*
- Ear, External/metabolism
- Ear, External/physiopathology*
- Female
- Gene Expression Profiling/methods
- Male
- Mammals/genetics
- Mammals/physiology
- Mice
- Murinae
- Rabbits
- Regeneration/genetics
- Regeneration/physiology*
- Species Specificity
- Wound Healing/genetics
- Wound Healing/physiology*
- PubMed
- 27109826 Full text @ Nat. Commun.
Citation
Gawriluk, T.R., Simkin, J., Thompson, K.L., Biswas, S.K., Clare-Salzler, Z., Kimani, J.M., Kiama, S.G., Smith, J.J., Ezenwa, V.O., Seifert, A.W. (2016) Comparative analysis of ear-hole closure identifies epimorphic regeneration as a discrete trait in mammals. Nature communications. 7:11164.
Abstract
Why mammals have poor regenerative ability has remained a long-standing question in biology. In regenerating vertebrates, injury can induce a process known as epimorphic regeneration to replace damaged structures. Using a 4-mm ear punch assay across multiple mammalian species, here we show that several Acomys spp. (spiny mice) and Oryctolagus cuniculus completely regenerate tissue, whereas other rodents including MRL/MpJ 'healer' mice heal similar injuries by scarring. We demonstrate ear-hole closure is independent of ear size, and closure rate can be modelled with a cubic function. Cellular and genetic analyses reveal that injury induces blastema formation in Acomys cahirinus. Despite cell cycle re-entry in Mus musculus and A. cahirinus, efficient cell cycle progression and proliferation only occurs in spiny mice. Together, our data unite blastema-mediated regeneration in spiny mice with regeneration in other vertebrates such as salamanders, newts and zebrafish, where all healthy adults regenerate in response to injury.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping