|ZFIN ID: ZDB-PUB-160414-16|
Proanthocyanidins, Isolated from Choerospondias axillaris Fruit Peels, Exhibit Potent Antioxidant Activities in Vitro and Novel Anti-angiogenic Property in Vitro and in Vivo
Li, Q., Wang, X., Dai, T., Liu, C., Li, T., McClements, D.J., Chen, J., Liu, J.
|Source:||Journal of Agricultural and Food Chemistry 64(18): 3546-56 (Journal)|
|Registered Authors:||Chen, Jun|
|Keywords:||Choerospondias axillaris, byproduct, proanthocyanidins, antioxidant, anti-angiogenesis|
|PubMed:||27066842 Full text @ J. Agric. Food Chem.|
Li, Q., Wang, X., Dai, T., Liu, C., Li, T., McClements, D.J., Chen, J., Liu, J. (2016) Proanthocyanidins, Isolated from Choerospondias axillaris Fruit Peels, Exhibit Potent Antioxidant Activities in Vitro and Novel Anti-angiogenic Property in Vitro and in Vivo. Journal of Agricultural and Food Chemistry. 64(18):3546-56.
ABSTRACTThe production of new blood vessels (angiogenesis) is an important stage in the growth and spread of cancerous tumors. Anti-angiogenesis is one strategy for controlling tumor progression. This study evaluated the antioxidant and antiangiogenic activities of a proanthocyanidins (PAs) extract from Choerospondias axillaris peels. HPLC-MS analysis revealed that numerous oligomeric forms of the PAs were detected in the PAs extract, including dimers, trimers, tetramers and flavan-3-ol monomers. The PAs extract possessed appreciable free radical scavenging activity (IC50/DPPH =164 ± 7 µg/mL, IC50/ABTS =154 ± 6 µg/mL), potent reducing power (0.930 ± 0.030 g AAE/g), and strong cellular antioxidant activity (EC50 =10.2 ± 1.4 and 38.9 ± 2.1 µg/mL without or with PBS wash, respectively). It could also retard various stages of angiogenesis, such as the migration of endothelial cells and the creation of tubes without causing toxicity to the cells. Regarding intracellular signal transduction, the PAs extract attenuated the phosphorylation of Akt, ERK, and p38MAPK dose-dependently in endothelial cells from human umbilical veins. In transgenic zebrafish embryo, new blood vessel formation was suppressed by PAs extract in a concentration-dependent manner at 72 h post fertilization. Thus, these results suggest that PAs from Choerospondias axillaris peels could be a good source of natural inhibitors to target angiogenesis.