PUBLICATION
Programming of the hypothalamic-pituitary-interrenal axis by maternal social status in zebrafish (Danio rerio)
- Authors
- Jeffrey, J.D., Gilmour, K.M.
- ID
- ZDB-PUB-160406-8
- Date
- 2016
- Source
- The Journal of experimental biology 219(Pt 11): 1734-43 (Journal)
- Registered Authors
- Keywords
- Dominance hierarchy, HPI axis, Fish, Offspring, Cortisol, Stress
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/physiology
- Embryonic Development/genetics
- Female
- Gene Expression Regulation, Developmental
- Hierarchy, Social*
- Hydrocortisone/metabolism
- Hypothalamo-Hypophyseal System/metabolism
- Hypothalamo-Hypophyseal System/physiology*
- Kidney/physiology*
- Pituitary-Adrenal System/metabolism
- Pituitary-Adrenal System/physiology*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction
- Stress, Physiological/genetics
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 27045091 Full text @ J. Exp. Biol.
Citation
Jeffrey, J.D., Gilmour, K.M. (2016) Programming of the hypothalamic-pituitary-interrenal axis by maternal social status in zebrafish (Danio rerio). The Journal of experimental biology. 219(Pt 11):1734-43.
Abstract
The present study examined the effects of maternal social status, with subordinate status being a chronic stressor, on development and activity of the stress axis in zebrafish embryos and larvae. Female zebrafish were confined in pairs for 48 h to establish dominant/subordinate hierarchies; their offspring were reared to 144 hours post-fertilization (hpf) and sampled at five time points over development. No differences were detected in maternal cortisol contribution, which is thought to be an important programmer of offspring phenotype. However, once zebrafish offspring began to synthesize cortisolde novo(48 hpf), larvae of dominant females exhibited significantly lower baseline cortisol levels than offspring of subordinate females. These lower cortisol levels may reflect reduced hypothalamic-pituitary-interrenal (HPI) axis activity, becausecorticotropin-releasing factor(crf) andcytochrome p450 side chain cleavage enzyme(p450scc) mRNA levels also were lower in larvae from dominant females. Moreover, baseline mRNA levels of HPI axis genes continued to be affected by maternal social status beyond 48 hpf. At 144 hpf, stress-induced cortisol levels were significantly lower in offspring of subordinate females. These results suggest programming of stress axis function in zebrafish offspring by maternal social status, emphasizing the importance of maternal environment and experience on offspring stress axis activity.
Errata / Notes
Correction published: Jeffrey, J.D., Gilmour, K. (2016) Correction: Programming of the hypothalamic-pituitary-interrenal axis by maternal social status in zebrafish (Danio rerio). J. Exp. Biol. 219: 2963.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping