PUBLICATION

Multicolor Cell Barcoding Technology for Long-Term Surveillance of Epithelial Regeneration in Zebrafish

Authors
Chen, C.H., Puliafito, A., Cox, B.D., Primo, L., Fang, Y., Di Talia, S., Poss, K.D.
ID
ZDB-PUB-160324-8
Date
2016
Source
Developmental Cell   36: 668-80 (Journal)
Registered Authors
Chen, Chen-Hui, Cox, Ben, Fang, Yi, Poss, Kenneth D.
Keywords
none
MeSH Terms
  • Animal Fins/injuries
  • Animal Fins/physiology
  • Animals
  • Animals, Genetically Modified
  • DNA Barcoding, Taxonomic/methods*
  • Epithelial Cells/physiology
  • Epithelium/injuries
  • Epithelium/physiology
  • Homeostasis
  • Models, Animal
  • Reactive Oxygen Species/metabolism
  • Regeneration/genetics
  • Regeneration/physiology
  • Skin/injuries
  • Skin Pigmentation/genetics
  • Skin Pigmentation/physiology
  • Zebrafish/genetics*
  • Zebrafish/physiology*
PubMed
27003938 Full text @ Dev. Cell
Abstract
Current fate mapping and imaging platforms are limited in their ability to capture dynamic behaviors of epithelial cells. To deconstruct regenerating adult epithelial tissue at single-cell resolution, we created a multicolor system, skinbow, that barcodes the superficial epithelial cell (SEC) population of zebrafish skin with dozens of distinguishable tags. With image analysis to directly segment and simultaneously track hundreds of SECs in vivo over entire surface lifetimes, we readily quantified the orchestration of cell emergence, growth, repositioning, and loss under homeostatic conditions and after exfoliation or appendage amputation. We employed skinbow-based imaging in conjunction with a live reporter of epithelial stem cell cycle activity and as an instrument to evaluate the effects of reactive oxygen species on SEC behavior during epithelial regeneration. Our findings introduce a platform for large-scale, quantitative in vivo imaging of regenerating skin and reveal unanticipated collective dynamism in epithelial cell size, mobility, and interactions.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping