PUBLICATION

Molecular Characterization and Expression Analyses of the Complement Component C8α, C8β and C9 Genes in Yellow Catfish (Pelteobagrus fulvidraco) after the Aeromonas hydrophila Challenge

Authors
Zheng, H., Ji, W., Zhang, G.R., Zhang, X.T., Shi, Z.C., Wei, K.J., Yang, R.B., Gardner, J.P.
ID
ZDB-PUB-160324-5
Date
2016
Source
International Journal of Molecular Sciences   17(3): 345 (Journal)
Registered Authors
Ji, Wei
Keywords
C8α, C8β, C9, Pelteobagrus fulvidraco, bacterial challenge, cDNA cloning, complement component, gene expression
MeSH Terms
  • Aeromonas hydrophila*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Catfishes/genetics
  • Catfishes/immunology*
  • Catfishes/microbiology
  • Complement C8/genetics*
  • Complement C9/genetics*
  • Fish Diseases/microbiology
  • Fish Proteins/genetics*
  • Gene Expression Regulation
  • Gram-Negative Bacterial Infections/immunology
  • Gram-Negative Bacterial Infections/veterinary
  • Organ Specificity
  • Phylogeny
  • Sequence Homology, Amino Acid
PubMed
27005612 Full text @ Int. J. Mol. Sci.
Abstract
The complement components C8α, C8β and C9 have important roles in the innate immune system against invading microorganisms. Partial cDNA sequences of the Pf_C8α, Pf_C8β and Pf_C9 genes (Pf: abbreviation of Pelteobagrus fulvidraco) were cloned from yellow catfish. The Pf_C8α, Pf_C8β and Pf_C9 genes showed the greatest amino acid similarity to C8α (54%) and C8β (62%) of zebrafish and to C9 (52%) of grass carp, respectively. Ontogenetic expression analyses using real-time quantitative PCR suggested that the three genes may play crucial roles during embryonic and early larval development. The mRNA expressions of the three genes were all at the highest levels in liver tissue, and at lower or much lower levels in 16 other tissues, demonstrating that the liver is the primary site for the protein synthesis of Pf_C8α, Pf_C8β and Pf_C9. Injection of Aeromonas hydrophila led to up-regulation of the three genes in the spleen, head kidney, kidney, liver and blood tissues, indicating that the three genes may contribute to the host's defense against invading pathogenic microbes. An increased understanding of the functions of the Pf_C8α, Pf_C8β and Pf_C9 genes in the innate immunity of yellow catfish will help enhance production of this valuable freshwater species.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping