PUBLICATION

Cardiac Light-Sheet Fluorescent Microscopy for Multi-Scale and Rapid Imaging of Architecture and Function

Authors
Fei, P., Lee, J., Packard, R.R., Sereti, K.I., Xu, H., Ma, J., Ding, Y., Kang, H., Chen, H., Sung, K., Kulkarni, R., Ardehali, R., Kuo, C.C., Xu, X., Ho, C.M., Hsiai, T.K.
ID
ZDB-PUB-160305-16
Date
2016
Source
Scientific Reports   6: 22489 (Journal)
Registered Authors
Xu, Xiaolei
Keywords
Biomedical engineering, Light-sheet microscopy
MeSH Terms
  • Algorithms*
  • Animals
  • Animals, Genetically Modified
  • Doxorubicin/pharmacology
  • Image Processing, Computer-Assisted/methods*
  • Mice
  • Microscopy, Fluorescence/methods
  • Myocardium/cytology*
  • Myocardium/metabolism*
  • Zebrafish/metabolism*
PubMed
26935567 Full text @ Sci. Rep.
Abstract
Light Sheet Fluorescence Microscopy (LSFM) enables multi-dimensional and multi-scale imaging via illuminating specimens with a separate thin sheet of laser. It allows rapid plane illumination for reduced photo-damage and superior axial resolution and contrast. We hereby demonstrate cardiac LSFM (c-LSFM) imaging to assess the functional architecture of zebrafish embryos with a retrospective cardiac synchronization algorithm for four-dimensional reconstruction (3-D space + time). By combining our approach with tissue clearing techniques, we reveal the entire cardiac structures and hypertrabeculation of adult zebrafish hearts in response to doxorubicin treatment. By integrating the resolution enhancement technique with c-LSFM to increase the resolving power under a large field-of-view, we demonstrate the use of low power objective to resolve the entire architecture of large-scale neonatal mouse hearts, revealing the helical orientation of individual myocardial fibers. Therefore, our c-LSFM imaging approach provides multi-scale visualization of architecture and function to drive cardiovascular research with translational implication in congenital heart diseases.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes