PUBLICATION
Direct visualization of replication dynamics in early zebrafish embryos
- Authors
- Kuriya, K., Higashiyama, E., Avşar-Ban, E., Okochi, N., Hattori, K., Ogata, S., Takebayashi, S.I., Ogata, M., Tamaru, Y., Okumura, K.
- ID
- ZDB-PUB-160301-7
- Date
- 2016
- Source
- Bioscience, biotechnology, and biochemistry 80(5): 945-8 (Journal)
- Registered Authors
- Tamaru, Yutaka
- Keywords
- DNA replication, replication foci, zebrafish embryo
- MeSH Terms
-
- Animals
- DNA Replication*
- Deoxyuridine/analogs & derivatives
- Deoxyuridine/metabolism
- Embryo, Nonmammalian/metabolism*
- Embryo, Nonmammalian/ultrastructure
- Embryonic Development/genetics*
- Microscopy, Fluorescence
- Staining and Labeling
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 26923175 Full text @ Biosci. Biotechnol. Biochem.
Citation
Kuriya, K., Higashiyama, E., Avşar-Ban, E., Okochi, N., Hattori, K., Ogata, S., Takebayashi, S.I., Ogata, M., Tamaru, Y., Okumura, K. (2016) Direct visualization of replication dynamics in early zebrafish embryos. Bioscience, biotechnology, and biochemistry. 80(5):945-8.
Abstract
We analyzed DNA replication in early zebrafish embryos. The replicating DNA of whole embryos was labeled with the thymidine analog 5-ethynyl-2'-deoxyuridine (EdU), and spatial regulation of replication sites was visualized in single embryo-derived cells. The results unveiled uncharacterized replication dynamics during zebrafish early embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping