PUBLICATION
Prophylactic administration of chicken cathelicidin-2 boosts zebrafish embryonic innate immunity
- Authors
- Schneider, V.A., van Dijk, A., van der Sar, A.M., Kraaij, M.D., Veldhuizen, E.J., Haagsman, H.P.
- ID
- ZDB-PUB-160228-7
- Date
- 2016
- Source
- Developmental and comparative immunology 60: 108-14 (Journal)
- Registered Authors
- van der Sar, Astrid M.
- Keywords
- Salmonella enteritidis, chicken cathelidicin-2, host defense peptide, immune stimulation, zebrafish embryos
- MeSH Terms
-
- Adjuvants, Immunologic/administration & dosage*
- Animals
- Antimicrobial Cationic Peptides/administration & dosage*
- Cell Proliferation
- Drug Evaluation, Preclinical
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/immunology
- Fish Diseases/immunology*
- Fish Diseases/microbiology
- Fish Diseases/prevention & control
- Immunity, Innate/drug effects*
- Phagocytes/physiology
- Salmonella Infections, Animal/immunology*
- Salmonella Infections, Animal/microbiology
- Salmonella Infections, Animal/prevention & control
- Salmonella enteritidis/immunology
- Zebrafish/immunology*
- PubMed
- 26920462 Full text @ Dev. Comp. Immunol.
Citation
Schneider, V.A., van Dijk, A., van der Sar, A.M., Kraaij, M.D., Veldhuizen, E.J., Haagsman, H.P. (2016) Prophylactic administration of chicken cathelicidin-2 boosts zebrafish embryonic innate immunity. Developmental and comparative immunology. 60:108-14.
Abstract
Chicken cathelicidin-2 (CATH-2) is a host defense peptide that exhibits immunomodulatory and antibacterial properties. Here we examined effects of CATH-2 in zebrafish embryos in the absence and presence of infection. Yolk-injection of 0.2-1.5 hours post-fertilized (hpf) zebrafish embryos with 2.6 ng/kg CATH-2 increased proliferation of phagocytic cells at 48 hpf by 30%. A lethal infection model was developed to test the prophylactic protective effect of CATH-2 peptide. Embryos (0.2-1.5 hpf) were injected with 2.6 ng/kg CATH-2, challenged with a lethal dose of fluorescently labeled S. enteritidis pGMDs3 at 28 hpf and monitored for survival. Prophylactic treatment with CATH-2 was found to delay infection starting at 22 hours post-infection (hpi). At 18-20 hpi, significantly lower (2-fold) fluorescence intensity and decreased bacterial loads were detected in peptide-treated embryos. Thus prophylactic administration of low CATH-2 concentrations confer partial protection in zebrafish embryos by boosting the innate immune system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping