PUBLICATION

Zebrafish and Medaka: new model organisms for modern biomedical research

Authors
Lin, C.Y., Chiang, C.Y., Tsai, H.J.
ID
ZDB-PUB-160130-5
Date
2016
Source
Journal of Biomedical Science   23: 19 (Review)
Registered Authors
Tsai, Huai-Jen
Keywords
Disease model organism, Developmental biology, Gene regulation, in vivo expression, Transgenic fish
MeSH Terms
  • Animals
  • Biomedical Research/methods*
  • Humans
  • Models, Biological*
  • Oryzias*/embryology
  • Oryzias*/genetics
  • Zebrafish*/embryology
  • Zebrafish*/genetics
PubMed
26822757 Full text @ J. Biomed. Sci.
Abstract
Although they are primitive vertebrates, zebrafish (Danio rerio) and medaka (Oryzias latipes) have surpassed other animals as the most used model organisms based on their many advantages. Studies on gene expression patterns, regulatory cis-elements identification, and gene functions can be facilitated by using zebrafish embryos via a number of techniques, including transgenesis, in vivo transient assay, overexpression by injection of mRNAs, knockdown by injection of morpholino oligonucleotides, knockout and gene editing by CRISPR/Cas9 system and mutagenesis. In addition, transgenic lines of model fish harboring a tissue-specific reporter have become a powerful tool for the study of biological sciences, since it is possible to visualize the dynamic expression of a specific gene in the transparent embryos. In particular, some transgenic fish lines and mutants display defective phenotypes similar to those of human diseases. Therefore, a wide variety of fish model not only sheds light on the molecular mechanisms underlying disease pathogenesis in vivo but also provides a living platform for high-throughput screening of drug candidates. Interestingly, transgenic model fish lines can also be applied as biosensors to detect environmental pollutants, and even as pet fish to display beautiful fluorescent colors. Therefore, transgenic model fish possess a broad spectrum of applications in modern biomedical research, as exampled in the following review.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes