PUBLICATION
A genetically targetable near-infrared photosensitizer
- Authors
- He, J., Wang, Y., Missinato, M.A., Onuoha, E., Perkins, L.A., Watkins, S.C., St Croix, C.M., Tsang, M., Bruchez, M.P.
- ID
- ZDB-PUB-160126-2
- Date
- 2016
- Source
- Nature Methods 13(3): 263-8 (Journal)
- Registered Authors
- Tsang, Michael, Wang, Yi
- Keywords
- Chemical tools, Optogenetics, Self-renewal
- MeSH Terms
-
- Apoptosis/physiology
- Apoptosis/radiation effects*
- Dose-Response Relationship, Radiation
- HEK293 Cells
- Humans
- Infrared Rays*
- Photochemotherapy/methods*
- Photosensitizing Agents/chemistry*
- Photosensitizing Agents/radiation effects*
- Radiation Dosage
- Recombinant Proteins/genetics*
- Recombinant Proteins/therapeutic use
- PubMed
- 26808669 Full text @ Nat. Methods
Citation
He, J., Wang, Y., Missinato, M.A., Onuoha, E., Perkins, L.A., Watkins, S.C., St Croix, C.M., Tsang, M., Bruchez, M.P. (2016) A genetically targetable near-infrared photosensitizer. Nature Methods. 13(3):263-8.
Abstract
Upon illumination, photosensitizer molecules produce reactive oxygen species that can be used for functional manipulation of living cells, including protein inactivation, targeted-damage introduction and cellular ablation. Photosensitizers used to date have been either exogenous, resulting in delivery and removal challenges, or genetically encoded proteins that form or bind a native photosensitizing molecule, resulting in a constitutively active photosensitizer inside the cell. We describe a genetically encoded fluorogen-activating protein (FAP) that binds a heavy atom-substituted fluorogenic dye, forming an 'on-demand' activated photosensitizer that produces singlet oxygen and fluorescence when activated with near-infrared light. This targeted and activated photosensitizer (TAPs) approach enables protein inactivation, targeted cell killing and rapid targeted lineage ablation in living larval and adult zebrafish. The near-infrared excitation and emission of this FAP-TAPs provides a new spectral range for photosensitizer proteins that could be useful for imaging, manipulation and cellular ablation deep within living organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping