ZFIN ID: ZDB-PUB-160119-2
Whole-brain activity mapping onto a zebrafish brain atlas
Randlett, O., Wee, C.L., Naumann, E.A., Nnaemeka, O., Schoppik, D., Fitzgerald, J.E., Portugues, R., Lacoste, A.M., Riegler, C., Engert, F., Schier, A.F.
Date: 2015
Source: Nature Methods   12: 1039-1046 (Journal)
Registered Authors: Engert, Florian, Randlett, Owen, Schier, Alexander, Schoppik, David
Keywords: Neuroscience, Zebrafish
MeSH Terms:
  • Algorithms
  • Animals
  • Automation
  • Behavior, Animal
  • Brain/metabolism*
  • Brain/physiology
  • Brain Mapping/methods
  • Calcium/chemistry
  • Extracellular Signal-Regulated MAP Kinases/metabolism*
  • Image Processing, Computer-Assisted/methods*
  • Immunohistochemistry
  • Microscopy, Confocal
  • Neurites/metabolism*
  • Neurons/metabolism
  • Neurons/physiology
  • Phosphorylation
  • Principal Component Analysis
  • Reproducibility of Results
  • Software
  • Swimming
  • Zebrafish
PubMed: 26778924 Full text @ Nat. Methods
In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open source atlas containing molecular labels and anatomical region definitions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated-Extracellular signal-regulated kinase (ERK/MAPK) as a readout of neural activity, we have developed a system to create and contextualize whole brain maps of stimulus- and behavior-dependent neural activity. This MAP-Mapping (Mitogen Activated Protein kinase - Mapping) assay is technically simple, fast, inexpensive, and data analysis is completely automated. Since MAP-Mapping is performed on fish that are freely swimming, it is applicable to nearly any stimulus or behavior. We demonstrate the utility of our high-throughput approach using hunting/feeding, pharmacological, visual and noxious stimuli. The resultant maps outline hundreds of areas associated with behaviors.