PUBLICATION
Whole-brain activity mapping onto a zebrafish brain atlas
- Authors
- Randlett, O., Wee, C.L., Naumann, E.A., Nnaemeka, O., Schoppik, D., Fitzgerald, J.E., Portugues, R., Lacoste, A.M., Riegler, C., Engert, F., Schier, A.F.
- ID
- ZDB-PUB-160119-2
- Date
- 2015
- Source
- Nature Methods 12: 1039-1046 (Journal)
- Registered Authors
- Engert, Florian, Randlett, Owen, Schier, Alexander, Schoppik, David
- Keywords
- Neuroscience, Zebrafish
- MeSH Terms
-
- Neurons/metabolism
- Neurons/physiology
- Brain/metabolism*
- Brain/physiology
- Zebrafish
- Behavior, Animal
- Software
- Automation
- Principal Component Analysis
- Extracellular Signal-Regulated MAP Kinases/metabolism*
- Image Processing, Computer-Assisted/methods*
- Algorithms
- Brain Mapping/methods
- Neurites/metabolism*
- Reproducibility of Results
- Phosphorylation
- Microscopy, Confocal
- Immunohistochemistry
- Calcium/chemistry
- Animals
- Swimming
- PubMed
- 26778924 Full text @ Nat. Methods
Citation
Randlett, O., Wee, C.L., Naumann, E.A., Nnaemeka, O., Schoppik, D., Fitzgerald, J.E., Portugues, R., Lacoste, A.M., Riegler, C., Engert, F., Schier, A.F. (2015) Whole-brain activity mapping onto a zebrafish brain atlas. Nature Methods. 12:1039-1046.
Abstract
In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open source atlas containing molecular labels and anatomical region definitions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated-Extracellular signal-regulated kinase (ERK/MAPK) as a readout of neural activity, we have developed a system to create and contextualize whole brain maps of stimulus- and behavior-dependent neural activity. This MAP-Mapping (Mitogen Activated Protein kinase - Mapping) assay is technically simple, fast, inexpensive, and data analysis is completely automated. Since MAP-Mapping is performed on fish that are freely swimming, it is applicable to nearly any stimulus or behavior. We demonstrate the utility of our high-throughput approach using hunting/feeding, pharmacological, visual and noxious stimuli. The resultant maps outline hundreds of areas associated with behaviors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping