ZFIN ID: ZDB-PUB-160117-8
Experimental Approaches to Study Endothelial Responses to Shear Stress.
Bowden, N., Bryan, M., Duckles, H., Feng, S., Hsiao, S., Kim, R., Mahmoud, M., Moers, B., Serbanovic-Canic, J., Ioannis, I., Ridger, V., Evans, P.
Date: 2016
Source: Antioxidants & redox signaling   25(7): 389-400 (Review)
Registered Authors: Kim, Rosemary
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Endothelial Cells/physiology*
  • Endothelium, Vascular/physiology*
  • Humans
  • In Vitro Techniques
  • Mechanotransduction, Cellular*
  • Stress, Mechanical*
PubMed: 26772071 Full text @ Antioxid. Redox Signal.
Shear stress controls multiple physiological processes in endothelial cells. This article describes some of the experimental techniques that can be used to study endothelial responses to shear stress. It includes an appraisal of large animal, rodent and zebrafish models of vascular mechanoresponsiveness. It also describes several bioreactors to apply flow to cells, and physical methods to separate mechanoresponses from mass transport mechanisms. We conclude that combining in vitro and in vivo approaches can provide a detailed mechanistic view of vascular responses to force, and that high throughput systems are required for unbiased assessment of the function of shear-induced molecules.