PUBLICATION
Glyphosate induces neurotoxicity in zebrafish
- Authors
- Roy, N.M., Carneiro, B., Ochs, J.
- ID
- ZDB-PUB-160117-2
- Date
- 2016
- Source
- Environmental Toxicology and Pharmacology 42: 45-54 (Journal)
- Registered Authors
- Roy, Nicole
- Keywords
- Development, Glyphosate, Neural, Roundup(®), Zebrafish
- MeSH Terms
-
- Animals
- Glycine/analogs & derivatives*
- Glycine/toxicity
- Herbicides/toxicity*
- Neurotoxins/toxicity*
- Toxicity Tests
- Water Pollutants, Chemical/toxicity
- Zebrafish/physiology
- PubMed
- 26773362 Full text @ Environ. Toxicol. Pharmacol.
Citation
Roy, N.M., Carneiro, B., Ochs, J. (2016) Glyphosate induces neurotoxicity in zebrafish. Environmental Toxicology and Pharmacology. 42:45-54.
Abstract
Glyphosate based herbicides (GBH) like Roundup(®) are used extensively in agriculture as well as in urban and rural settings as a broad spectrum herbicide. Its mechanism of action was thought to be specific only to plants and thus considered safe and non-toxic. However, mounting evidence suggests that GBHs may not be as safe as once thought as initial studies in frogs suggest that GBHs may be teratogenic. Here we utilize the zebrafish vertebrate model system to study early effects of glyphosate exposure using technical grade glyphosate and the Roundup(®) Classic formulation. We find morphological abnormalities including cephalic and eye reductions and a loss of delineated brain ventricles. Concomitant with structural changes in the developing brain, using in situ hybridization analysis, we detect decreases in genes expressed in the eye, fore and midbrain regions of the brain including pax2, pax6, otx2 and ephA4. However, we do not detect changes in hindbrain expression domains of ephA4 nor exclusive hindbrain markers krox-20 and hoxb1a. Additionally, using a Retinoic Acid (RA) mediated reporter transgenic, we detect no alterations in the RA expression domains in the hindbrain and spinal cord, but do detect a loss of expression in the retina. We conclude that glyphosate and the Roundup(®) formulation is developmentally toxic to the forebrain and midbrain but does not affect the hindbrain after 24h exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping