PUBLICATION

Pbx4 is Required for the Temporal Onset of Zebrafish Myocardial Differentiation

Authors
Kao, R.M., Rurik, J.G., Farr, G.H., Dong, X.R., Majesky, M.W., Maves, L.
ID
ZDB-PUB-160116-5
Date
2015
Source
Journal of developmental biology   3: 93-111 (Journal)
Registered Authors
Farr III, G. Hank, Maves, Lisa
Keywords
cardiomyocyte, differentiation, myocardial morphogenesis, outflow tract, pbx4, proepicardium, zebrafish
MeSH Terms
none
PubMed
26770887 Full text @ J Dev Biol
Abstract
Proper control of the temporal onset of cellular differentiation is critical for regulating cell lineage decisions and morphogenesis during development. Pbx homeodomain transcription factors have emerged as important regulators of cellular differentiation. We previously showed, by using antisense morpholino knockdown, that Pbx factors are needed for the timely activation of myocardial differentiation in zebrafish. In order to gain further insight into the roles of Pbx factors in heart development, we show here that zebrafish pbx4 mutant embryos exhibit delayed onset of myocardial differentiation, such as delayed activation of tnnt2a expression in early cardiomyocytes in the anterior lateral plate mesoderm. We also observe delayed myocardial morphogenesis and dysmorphic patterning of the ventricle and atrium, consistent with our previous Pbx knock-down studies. In addition, we find that pbx4 mutant larvae have aberrant outflow tracts and defective expression of the proepicardial marker tbx18. Finally, we present evidence for Pbx expression in cardiomyocyte precursors as well as heterogeneous Pbx expression among the pan-cytokeratin-expressing proepicardial cells near the developing ventricle. In summary, our data show that Pbx4 is required for the proper temporal activation of myocardial differentiation and establish a basis for studying additional roles of Pbx factors in heart development.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
pbx4b557/b557 (AB)controlPrim-5
pbx4b557/b557 (AB)controlPrim-5
pbx4b557/b557 (AB)controlPrim-5
pbx4b557/b557 (AB)controlLong-pec
pbx4b557/b557 (AB)controlLong-pec
pbx4b557/b557 (AB)controlLong-pec
pbx4b557/b557 (AB)controlProtruding-mouth
pbx4b557/b557 (AB)controlProtruding-mouth
pbx4b557/b557 (AB)standard conditions10-13 somites
pbx4b557/b557 (AB)standard conditions10-13 somites
1 - 10 of 28
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b557
    		Point Mutation
    s958TgTransgenic Insertion
      sd12TgTransgenic Insertion
        twu34TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          Fish
          AB
          pbx4b557/b557 (AB)
          pbx4b557/b557; s958Tg; sd12Tg
          pbx4b557/b557; sd12Tg
          pbx4b557/b557; twu34Tg
          s958Tg; sd12Tg
          sd12Tg
          twu34Tg
          1 - 8 of 8
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          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          mCherryEFGmCherry
          1 - 2 of 2
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          Mapping
          No data available
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          Citation
          Kao, R.M., Rurik, J.G., Farr, G.H., Dong, X.R., Majesky, M.W., Maves, L. (2015) Pbx4 is Required for the Temporal Onset of Zebrafish Myocardial Differentiation. Journal of developmental biology. 3:93-111.