PUBLICATION
Reverse Genetics in Zebrafish: Mutants, Morphants, and Moving Forward
- Authors
- Lawson, N.D.
- ID
- ZDB-PUB-160108-5
- Date
- 2016
- Source
- Trends in cell biology 26(2): 77-9 (Review)
- Registered Authors
- Lawson, Nathan
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Gene Knockdown Techniques/methods
- Gene Knockdown Techniques/trends
- Humans
- Morpholinos/genetics
- Mutagenesis/physiology
- Mutation/genetics
- Reverse Genetics*/methods
- Reverse Genetics*/trends
- Zebrafish/genetics*
- PubMed
- 26739910 Full text @ Trends Cell Biol.
Citation
Lawson, N.D. (2016) Reverse Genetics in Zebrafish: Mutants, Morphants, and Moving Forward. Trends in cell biology. 26(2):77-9.
Abstract
Gene editing in zebrafish has begun to reveal discordance between mutant phenotypes and those associated with knockdown via morpholino oligonucleotides (MOs). These studies suggest that MOs should not be used as a standalone tool and underscore the need for guidelines that require defined mutants to assess gene function in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping