PUBLICATION
Neurobehavioral impairments produced by developmental lead exposure persisted for generations in zebrafish (Danio rerio)
- Authors
- Xu, X., Weber, D., Burge, R., VanAmberg, K.
- ID
- ZDB-PUB-151222-3
- Date
- 2016
- Source
- Neurotoxicology 52: 176-85 (Journal)
- Registered Authors
- Weber, Dan
- Keywords
- Active avoidance conditioning, Embryonic, Lead (Pb), Learning, Trans-generational, Zebrafish
- MeSH Terms
-
- Animals
- Avoidance Learning/drug effects
- Behavior, Animal/drug effects*
- Cohort Effect*
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects
- Female
- Lead/toxicity*
- Male
- Nitrates/toxicity*
- Zebrafish/embryology*
- PubMed
- 26688331 Full text @ Neurotoxicology
Citation
Xu, X., Weber, D., Burge, R., VanAmberg, K. (2016) Neurobehavioral impairments produced by developmental lead exposure persisted for generations in zebrafish (Danio rerio). Neurotoxicology. 52:176-85.
Abstract
The zebrafish has become a useful animal model for studying the effects of environmental contaminants on neurobehavioral development due to its ease of breeding, high number of eggs per female, short generation times, and a well-established avoidance conditioning paradigm. Using avoidance conditioning as the behavioral paradigm, the present study investigated the effects of embryonic exposure to lead (Pb) on learning in adult zebrafish and the third (F3) generation of those fish. In Experiment 1, adult zebrafish that were developmentally exposed to 0.0, 0.1, 1.0 or 10.0μM Pb (2-24 hr post fertilization) as embryos were trained and tested for avoidance responses. The results showed that adult zebrafish hatched from embryos exposed to 0.0 or 0.1μM Pb learned avoidance responses during training and displayed significantly increased avoidance responses during testing, while those hatched from embryos exposed to 1.0 or 10.0μM Pb displayed no significant increases in avoidance responses from training to testing. In Experiment 2, the F3 generation of zebrafish that were developmentally exposed to an identical exposure regimen as in Experiment 1 were trained and tested for avoidance responses. The results showed that the F3 generation of zebrafish developmentally exposed as embryos to 0.0 or 0.1μM Pb learned avoidance responses during training and displayed significantly increased avoidance responses during testing, while the F3 generation of zebrafish developmentally exposed as embryos to 1.0 or 10.0μM Pb displayed no significant changes in avoidance responses from training to testing. Thus, developmental Pb exposure produced learning impairments that persisted for at least three generations, demonstrating trans-generational effects of embryonic exposure to Pb.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping