PUBLICATION
Comparative Proteomic, Physiological, Morphological, and Biochemical Analyses Reveal the Characteristics of the Diploid Spermatozoa of Allotetraploid Hybrids of Red Crucian Carp (Carassius auratus) and Common Carp (Cyprinus carpio)
- Authors
- Duan, W., Xu, K., Hu, F., Zhang, Y., Wen, M., Wang, J., Tao, M., Luo, K., Zhao, R., Qin, Q., Zhang, C., Liu, J., Liu, Y., Liu, S.
- ID
- ZDB-PUB-151218-10
- Date
- 2016
- Source
- Biology of reproduction 94(2): 35 (Journal)
- Registered Authors
- Wang, Jing
- Keywords
- Comparative reproduction, Fish reproduction, Gamete biology, Male reproductive tract, Sperm motility and transport
- MeSH Terms
-
- Animals
- Carps/physiology*
- Diploidy*
- Male
- Polyploidy*
- Proteomics
- Spermatozoa/cytology
- Spermatozoa/physiology*
- Testis/cytology
- Testis/physiology*
- PubMed
- 26674567 Full text @ Biol. Reprod.
Citation
Duan, W., Xu, K., Hu, F., Zhang, Y., Wen, M., Wang, J., Tao, M., Luo, K., Zhao, R., Qin, Q., Zhang, C., Liu, J., Liu, Y., Liu, S. (2016) Comparative Proteomic, Physiological, Morphological, and Biochemical Analyses Reveal the Characteristics of the Diploid Spermatozoa of Allotetraploid Hybrids of Red Crucian Carp (Carassius auratus) and Common Carp (Cyprinus carpio). Biology of reproduction. 94(2):35.
Abstract
The generation of diploid spermatozoa is essential for the continuity of tetraploid lineages. The DNA content of diploid spermatozoa from allotetraploid hybrids of red crucian carp and common carp was nearly twice as great as that of haploid spermatozoa from common carp, and the durations of rapid and slow progressive motility were longer. We performed comparative proteomic analyses to measure variations in protein composition between diploid and haploid spermatozoa. Using two-dimensional electrophoresis followed by LC-MS/MS, 21 protein spots that changed in abundance were analyzed. As common carp and the allotetraploid hybrids are not fully sequenced organisms, we identified proteins by Mascot searching against the NCBI nr protein database for the zebrafish (Danio rerio), and verified them against predicted homologous proteins derived from transcriptomes of the testis. Twenty protein spots were identified successfully, belonging to four gene ontogeny categories: cytoskeleton, energy metabolism, the ubiquitin--proteasome system and other functions, indicating that these might be associated with the variation in diploid spermatozoa. This categorization of variations in protein composition in diploid spermatozoa will provide new perspectives on male polyploidy. Further work is required to define the functions of each protein. Moreover, our approach indicates that transcriptome data are useful for proteomic analyses in organisms lacking full protein sequences.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping