ZFIN ID: ZDB-PUB-151208-4
Understanding and Editing the Zebrafish Genome
Varshney, G.K., Sood, R., Burgess, S.M.
Date: 2015
Source: Advances in genetics   92: 1-52 (Review)
Registered Authors: Burgess, Shawn, Sood, Raman, Varshney, Gaurav
Keywords: CRISPR/Cas9, Genomics, Resources, Tal-effector nucleases, Targeted mutagenesis, Zebrafish, Zinc finger nucleases
MeSH Terms:
  • Animals
  • CRISPR-Cas Systems
  • Gene Knock-In Techniques
  • Gene Knockout Techniques
  • Gene Targeting/methods*
  • Genetic Engineering/methods*
  • Genome
  • Models, Animal
  • Zebrafish/genetics*
PubMed: 26639914 Full text @ Adv. Genet.
In the last two decades, zebrafish has become one of the fastest growing model organisms in terms of publications, however it has been plagued with the absence of a key tool in the genetics toolbox: the ability to systematically make targeted mutations in the genome. That all changed with the recent emergence of custom-built, sequence-specific nucleases, i.e., zinc finger nucleases (ZFNs), TAL-effector nucleases (TALENs), or clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas9. Here, we provide a comprehensive review of the application of these genome-editing tools to generate targeted knockout and knock-in mutants in zebrafish. These technologies have allowed us to transition from targeted knockouts in zebrafish being a difficult, resource intensive undertaking, to something that can be done in virtually any lab with modest molecular biology experience. Furthermore, this review provides a comprehensive listing of genetic and genomic resources and online tools that zebrafish researchers can use to help find a desired mutation or design effective ZFNs, TALENs, or CRISPR guide RNAs for their targeting experiments.