PUBLICATION
Interaction between handling induced stress and anxiolytic effects of ethanol in zebrafish: A behavioral and neurochemical analysis
- Authors
- Tran, S., Nowicki, M., Fulcher, N., Chatterjee, D., Gerlai, R.
- ID
- ZDB-PUB-151128-7
- Date
- 2016
- Source
- Behavioural brain research 298(Pt B): 278-85 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- Zebrafish, alcohol, dopamine, serotonin, stress
- MeSH Terms
-
- 3,4-Dihydroxyphenylacetic Acid/metabolism
- Animals
- Anti-Anxiety Agents/pharmacology*
- Anxiety Disorders/drug therapy
- Anxiety Disorders/etiology
- Anxiety Disorders/metabolism
- Brain/drug effects
- Brain/metabolism
- Chromatography, High Pressure Liquid
- Disease Models, Animal*
- Dopamine/metabolism
- Ethanol/pharmacology*
- Female
- Handling, Psychological*
- Hydroxyindoleacetic Acid/metabolism
- Male
- Motor Activity/drug effects
- Serotonin/metabolism
- Stress, Psychological/drug therapy*
- Stress, Psychological/etiology
- Stress, Psychological/metabolism
- Time
- Zebrafish/metabolism*
- PubMed
- 26611561 Full text @ Behav. Brain Res.
Citation
Tran, S., Nowicki, M., Fulcher, N., Chatterjee, D., Gerlai, R. (2016) Interaction between handling induced stress and anxiolytic effects of ethanol in zebrafish: A behavioral and neurochemical analysis. Behavioural brain research. 298(Pt B):278-85.
Abstract
Stress is often considered an important factor in the development of alcohol addiction. In rodents, various types of stressors have been shown to potentiate the effects of alcohol on behavioral responses, and to increase consumption of this substance. However, few have investigated the interaction between stress and alcohol in zebrafish. In the current study we present a repeated handling stress paradigm we developed for zebrafish, and examine whether stress alters alcohol induced behavioral and neurochemical responses. Our results show that repeated handling of zebrafish conducted for 2 consecutive days is sufficient to increase anxiety-like behavioral responses quantified 24hours post-stressor. Repeatedly handled zebrafish also exhibited a reduction in the levels of serotonin's metabolite, 5-hydroxyindole acetic acid (quantified by high precision liquid chromatography) compared to unhandled controls. A 60-minute acute exposure to 1% ethanol was found to significantly increase locomotor activity and decrease anxiety-like behavioral responses in stressed zebrafish but not in controls. Furthermore, unhandled control zebrafish exhibited a significant increase in whole-brain dopamine levels following exposure to ethanol but the increase was not observed in repeatedly handled fish. Our findings suggest that ethanol induced locomotor activity and anxiolysis is potentiated by handling stress and may be partially mediated by changes in dopaminergic and serotonergic activity. Overall, we demonstrate the validity of our repeated handling stressor paradigm for zebrafish, which can be used to investigate the interaction between stress and ethanol.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping