PUBLICATION
Mapping of Brain lipid binding protein (Blbp) in the brain of adult zebrafish, co-expression with aromatase B and links with proliferation
- Authors
- Diotel, N., Vaillant, C., Kah, O., Pellegrini, E.
- ID
- ZDB-PUB-151128-6
- Date
- 2016
- Source
- Gene expression patterns : GEP 20(1): 42-54 (Journal)
- Registered Authors
- Diotel, Nicolas, Kah, Olivier
- Keywords
- Blbp, aromatase B, estrogen, neurogenesis, radial glial cells, zebrafish
- MeSH Terms
-
- Animals
- Brain/metabolism*
- Male
- Brain Mapping
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Female
- Zebrafish/metabolism
- Neuroglia/cytology
- Neuroglia/metabolism
- Fatty Acid-Binding Proteins/genetics*
- Fatty Acid-Binding Proteins/metabolism
- Aromatase/genetics*
- Aromatase/metabolism
- Cell Proliferation
- PubMed
- 26611722 Full text @ Gene Expr. Patterns
Citation
Diotel, N., Vaillant, C., Kah, O., Pellegrini, E. (2016) Mapping of Brain lipid binding protein (Blbp) in the brain of adult zebrafish, co-expression with aromatase B and links with proliferation. Gene expression patterns : GEP. 20(1):42-54.
Abstract
Adult fish exhibit a strong neurogenic capacity due to the persistence of radial glial progenitor cells. In zebrafish, radial glial cells display well-established markers such as the estrogen-synthesizing enzyme (AroB) and the brain lipid binding protein (Blbp), which is known to strongly bind omega-3 polyunsaturated fatty acids such as docosahexaenoic acid (DHA). While Blpb is mainly described in the telencephalon of adult zebrafish, its expression in the remaining regions of the brain is poorly documented. The present study was designed to further investigate Blbp expression in the brain, its co-expression with AroB, and its link with radial glial cells proliferation in zebrafish. We generated a complete and detailed mapping of Blbp expression in the whole brain and show its complete co-expression with AroB, except in some tectal and hypothalamic regions. By performing PCNA and Blbp immunohistochemistry on cyp19a1b-GFP (AroB-GFP) fish, we also demonstrated preferential Blbp expression in proliferative radial glial cells in almost all regions studied. To our knowledge, this is the first complete and detailed mapping of Blbp-expressing cells showing strong association between Blbp and radial glial cell proliferation in the adult brain of fish. Given that zebrafish is now recognized models for studying neurogenesis and brain repair, our data provide detailed characterization of Blbp in the entire brain and open up a broad field of research investigating the role of omega-3 polyunsaturated fatty acids in neural stem cell activity in fish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping