PUBLICATION

Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model

Authors
Gorelick, D.A., Pinto, C.L., Hao, R., Bondesson, M.
ID
ZDB-PUB-151121-11
Date
2016
Source
Methods in molecular biology (Clifton, N.J.)   1366: 315-25 (Chapter)
Registered Authors
Gorelick, Daniel
Keywords
Estrogen, Fluorescence microscopy, Image analysis, Transgenic zebrafish, Xenoestrogens
MeSH Terms
  • Receptors, Estrogen/drug effects*
  • Receptors, Estrogen/genetics
  • Receptors, Estrogen/metabolism
  • Estrogen Antagonists/pharmacology
  • Animals
  • Response Elements
  • Microscopy, Fluorescence
  • Endocrine Disruptors/pharmacology*
  • Dose-Response Relationship, Drug
  • Ligands
  • Green Fluorescent Proteins/genetics*
  • Green Fluorescent Proteins/metabolism
  • Estrogens/pharmacology*
  • Animals, Genetically Modified
  • Workflow
  • Time Factors
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Gene Expression Regulation
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Signal Transduction/drug effects
  • Genes, Reporter*
PubMed
26585145 Full text @ Meth. Mol. Biol.
Abstract
In vivo models to detect estrogenic compounds are very valuable for screening for endocrine disruptors. Here we describe the use of transgenic estrogen reporter zebrafish as an in vivo model for identification of estrogenic properties of compounds. Live imaging of these transgenic fish provides knowledge of estrogen receptor specificity of different ligands as well as dynamics of estrogen signaling. Coupled to image analysis, the model can provide quantitative dose-response information on estrogenic activity of chemical compounds.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping