PUBLICATION
Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model
- Authors
- Gorelick, D.A., Pinto, C.L., Hao, R., Bondesson, M.
- ID
- ZDB-PUB-151121-11
- Date
- 2016
- Source
- Methods in molecular biology (Clifton, N.J.) 1366: 315-25 (Chapter)
- Registered Authors
- Gorelick, Daniel
- Keywords
- Estrogen, Fluorescence microscopy, Image analysis, Transgenic zebrafish, Xenoestrogens
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Endocrine Disruptors/pharmacology*
- Estrogen Antagonists/pharmacology
- Estrogens/pharmacology*
- Gene Expression Regulation
- Genes, Reporter*
- Green Fluorescent Proteins/genetics*
- Green Fluorescent Proteins/metabolism
- Ligands
- Microscopy, Fluorescence
- Receptors, Estrogen/drug effects*
- Receptors, Estrogen/genetics
- Receptors, Estrogen/metabolism
- Response Elements
- Signal Transduction/drug effects
- Time Factors
- Workflow
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 26585145 Full text @ Meth. Mol. Biol.
Citation
Gorelick, D.A., Pinto, C.L., Hao, R., Bondesson, M. (2016) Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model. Methods in molecular biology (Clifton, N.J.). 1366:315-25.
Abstract
In vivo models to detect estrogenic compounds are very valuable for screening for endocrine disruptors. Here we describe the use of transgenic estrogen reporter zebrafish as an in vivo model for identification of estrogenic properties of compounds. Live imaging of these transgenic fish provides knowledge of estrogen receptor specificity of different ligands as well as dynamics of estrogen signaling. Coupled to image analysis, the model can provide quantitative dose-response information on estrogenic activity of chemical compounds.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping