PUBLICATION
Effects of embryonic exposure to polychlorinated biphenyls (PCBs) on larval zebrafish behavior
- Authors
- Lovato, A.K., Creton, R., Colwill, R.M.
- ID
- ZDB-PUB-151114-11
- Date
- 2016
- Source
- Neurotoxicology and teratology 53: 1-10 (Journal)
- Registered Authors
- Creton, Robbert
- Keywords
- Avoidance behavior, Freezing, Larvae, Polychlorinated biphenyls, Thigmotaxis, Zebrafish
- MeSH Terms
-
- Analysis of Variance
- Animals
- Antithyroid Agents/pharmacology*
- Avoidance Learning/drug effects*
- Chlorodiphenyl (54% Chlorine)/pharmacology*
- Dose-Response Relationship, Drug
- Freezing Reaction, Cataleptic/drug effects*
- Larva/drug effects*
- Larva/physiology
- Motor Activity/drug effects*
- Photic Stimulation
- Time Factors
- Zebrafish/embryology
- PubMed
- 26561944 Full text @ Neurotoxicol. Teratol.
Citation
Lovato, A.K., Creton, R., Colwill, R.M. (2016) Effects of embryonic exposure to polychlorinated biphenyls (PCBs) on larval zebrafish behavior. Neurotoxicology and teratology. 53:1-10.
Abstract
Developmental disorders such as anxiety, autism, and attention deficit hyperactivity disorders have been linked to exposure to polychlorinated biphenyls (PCBs), a ubiquitous anthropogenic pollutant. The zebrafish is widely recognized as an excellent model system for assessing the effects of toxicant exposure on behavior and neurodevelopment. In the present study, we examined the effect of sub-chronic embryonic exposure to the PCB mixture, Aroclor (A) 1254 on anxiety-related behaviors in zebrafish larvae at 7days post-fertilization (dpf). We found that exposure to low concentrations of A1254, from 2 to 26h post-fertilization (hpf) induced specific behavioral defects in two assays. In one assay with intermittent presentations of a moving visual stimulus, 5ppm and 10ppm PCB-exposed larvae displayed decreased avoidance behavior but no significant differences in thigmotaxis or freezing relative to controls. In the other assay with intermittent presentations of a moving visual stimulus and a stationary visual stimulus, 5ppm and 10ppm PCB-exposed larvae had elevated baseline levels of thigmotaxis but no significant differences in avoidance behavior relative to controls. The 5ppm larvae also displayed higher terminal levels of freezing relative to controls. Collectively, our results show that exposure to ecologically valid PCB concentrations during embryonic development can induce functional deficits and alter behavioral responses to a visual threat.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping