ZFIN ID: ZDB-PUB-151104-4
Cortical depth and differential transport of vegetally localized dorsal and germ line determinants in the zebrafish embryo
Welch, E., Pelegri, F.
Date: 2014
Source: Bioarchitecture   5(1-2): 13-26 (Journal)
Registered Authors: Pelegri, Francisco
Keywords: Axis induction, RNA localization, cortical rotation, embryo, germ cell determination, germ plasm, vegetal cortex, zebrafish
MeSH Terms:
  • Actin Cytoskeleton/drug effects
  • Actin Cytoskeleton/metabolism
  • Actin Cytoskeleton/ultrastructure
  • Animals
  • Biological Transport
  • Body Patterning/genetics*
  • Bridged Bicyclo Compounds, Heterocyclic/pharmacology
  • Carrier Proteins/genetics*
  • Carrier Proteins/metabolism
  • Cytoskeletal Proteins/genetics*
  • Cytoskeletal Proteins/metabolism
  • Embryo, Nonmammalian
  • Female
  • Fertilization
  • Gene Expression Regulation, Developmental
  • In Situ Hybridization, Fluorescence
  • Male
  • Microtubules/drug effects
  • Microtubules/metabolism
  • Microtubules/ultrastructure
  • Nocodazole/pharmacology
  • Oocytes/drug effects
  • Oocytes/growth & development
  • Oocytes/metabolism
  • Oocytes/ultrastructure
  • RNA, Messenger/genetics*
  • RNA, Messenger/metabolism
  • RNA-Binding Proteins/genetics*
  • RNA-Binding Proteins/metabolism
  • Receptors, AMPA/genetics*
  • Receptors, AMPA/metabolism
  • Signal Transduction
  • Thiazolidines/pharmacology
  • Tubulin Modulators/pharmacology
  • Wnt Proteins/genetics*
  • Wnt Proteins/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zygote/drug effects
  • Zygote/growth & development
  • Zygote/metabolism
  • Zygote/ultrastructure
PubMed: 26528729 Full text @ Bioarchitecture
In zebrafish embryos, factors involved in both axis induction and primordial germ cell (PGC) development are localized to the vegetal pole of the egg. However, upon egg activation axis induction factors experience an asymmetric off-center shift whereas PGC factors undergo symmetric animally-directed movement. We examined the spatial relationship between the proposed dorsal genes wnt8a and grip2a and the PGC factor dazl at the vegetal cortex. We find that RNAs for these genes localize to different cortical depths, with the RNA for the PGC factor dazl at a deeper cortical level than those for axis-inducing factors. In addition, and in contrast to the role of microtubules in the long-range transport of dorsal determinants, we find that germ line determinant transport depends on the actin cytoskeleton. Our results support a model in which vegetal cortex differential RNA transport behavior is facilitated by RNA localization along cortical depth and differential coupling to cortical transport.