ZFIN ID: ZDB-PUB-151022-4
A role for nitric oxide in the control of breathing in zebrafish (Danio rerio)
Porteus, C.S., Pollack, J., Tzaneva, V., Kwong, R.W., Kumai, Y., Abdallah, S.J., Zaccone, G., Lauriano, E.R., Milsom, W.K., Perry, S.F.
Date: 2015
Source: The Journal of experimental biology   218(Pt 23): 3746-53 (Journal)
Registered Authors: Perry, Steve F.
Keywords: Chemoreceptor, hypoxia, Hyperoxia, Immunohistochemistry, Neuroepithelial cell, Morpholino
MeSH Terms:
  • Animals
  • Cell Hypoxia
  • Chemoreceptor Cells/physiology
  • Gills/physiology
  • Larva/physiology
  • NG-Nitroarginine Methyl Ester/pharmacology
  • Neuroepithelial Cells/drug effects
  • Neuroepithelial Cells/physiology
  • Nitric Oxide/physiology*
  • Nitric Oxide Donors/pharmacology
  • Nitric Oxide Synthase Type I/analysis
  • Nitroprusside/pharmacology
  • Oxygen/metabolism*
  • Zebrafish/physiology*
PubMed: 26486367 Full text @ J. Exp. Biol.
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ABSTRACT
Nitric oxide (NO) is a gaseous neurotransmitter, which in adult mammals, modulates the acute hypoxic ventilatory response; its role in the control of breathing in fish during development is unknown. We addressed the interactive effects of developmental age and NO in the control of piscine breathing by measuring the ventilatory response of zebrafish (Danio rerio) adults and larvae to NO donors and by inhibiting endogenous production of NO. In adults, sodium nitroprusside (SNP), a NO donor, inhibited ventilation; the extent of the ventilatory inhibition was related to the pre-existing ventilatory drive, with the greatest inhibition exhibited during exposure to hypoxia (PO2=5.6 kPa). Inhibition of endogenous NO production using L-NAME supressed the hypoventilatory response to hyperoxia, supporting an inhibitory role of NO in adult zebrafish. Neuroepithelial cells, the putative oxygen chemoreceptors of fish, contain neuronal nitric oxide synthase (nNOS). In zebrafish larvae at 4 days post fertilization, SNP increased ventilation in a concentration-dependent manner. Inhibition of NOS activity with L-NAME or knockdown of nNOS inhibited the hypoxic (PO2=3.5 kPa) ventilatory response. Immunohistochemistry revealed the presence of nNOS in the NECs of larvae. Taken together, these data suggest that NO plays an inhibitory role in the control ventilation in adult zebrafish, but an excitatory role in larvae.
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