PUBLICATION
Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways
- Authors
- Liang, F., Han, Y., Gao, H., Xin, S., Chen, S., Wang, N., Qin, W., Zhong, H., Lin, S., Yao, X., Li, S.
- ID
- ZDB-PUB-151009-3
- Date
- 2015
- Source
- Scientific Reports 5: 14468 (Journal)
- Registered Authors
- Lin, Shuo, Zhong, Hanbing
- Keywords
- none
- MeSH Terms
-
- Medicine, Chinese Traditional
- Animals, Genetically Modified
- Plants, Medicinal
- Fibroblast Growth Factors/antagonists & inhibitors
- Fibroblast Growth Factors/genetics
- Fibroblast Growth Factors/metabolism
- Animals
- Humans
- Human Umbilical Vein Endothelial Cells
- Zebrafish
- Kaempferols/isolation & purification
- Kaempferols/pharmacology*
- Chemical Fractionation/methods
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Neovascularization, Physiologic/drug effects*
- Vascular Endothelial Growth Factor Receptor-2/antagonists & inhibitors
- Vascular Endothelial Growth Factor Receptor-2/genetics
- Vascular Endothelial Growth Factor Receptor-2/metabolism
- Gene Expression Regulation
- Angiogenesis Inhibitors/isolation & purification
- Angiogenesis Inhibitors/pharmacology*
- Berberidaceae/chemistry*
- Small Molecule Libraries/isolation & purification
- Small Molecule Libraries/pharmacology*
- Cell Proliferation/drug effects
- Biological Assay
- Cell Movement/drug effects
- Embryo, Nonmammalian
- Plant Extracts/chemistry
- PubMed
- 26446489 Full text @ Sci. Rep.
Citation
Liang, F., Han, Y., Gao, H., Xin, S., Chen, S., Wang, N., Qin, W., Zhong, H., Lin, S., Yao, X., Li, S. (2015) Kaempferol Identified by Zebrafish Assay and Fine Fractionations Strategy from Dysosma versipellis Inhibits Angiogenesis through VEGF and FGF Pathways. Scientific Reports. 5:14468.
Abstract
Natural products are a rich resource for the discovery of therapeutic substances. By directly using 504 fine fractions from isolated traditional Chinese medicine plants, we performed a transgenic zebrafish based screen for anti-angiogenesis substances. One fraction, DYVE-D3, was found to inhibit the growth of intersegmental vessels in the zebrafish vasculature. Bioassay-guided isolation of DYVE-D3 indicates that the flavonoid kaempferol was the active substance. Kaempferol also inhibited the proliferation and migration of HUVECs in vitro. Furthermore, we found that kaempferol suppressed angiogenesis through inhibiting VEGFR2 expression, which can be enhanced by FGF inhibition. In summary, this study shows that the construction of fine fraction libraries allows efficient identification of active substances from natural products.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping