PUBLICATION
Neurochemical measurements in the zebrafish brain
- Authors
- Jones, L.J., McCutcheon, J.E., Young, A.M., Norton, W.H.
- ID
- ZDB-PUB-151007-3
- Date
- 2015
- Source
- Frontiers in behavioral neuroscience 9: 246 (Journal)
- Registered Authors
- Norton, Will
- Keywords
- 5-HT, adult brain, dopamine, fast scan cyclic voltammetry, histamine, neurochemistry, pH, zebrafish
- MeSH Terms
- none
- PubMed
- 26441575 Full text @ Front. Behav. Neurosci.
Citation
Jones, L.J., McCutcheon, J.E., Young, A.M., Norton, W.H. (2015) Neurochemical measurements in the zebrafish brain. Frontiers in behavioral neuroscience. 9:246.
Abstract
The zebrafish is an ideal model organism for behavioral genetics and neuroscience. The high conservation of genes and neurotransmitter pathways between zebrafish and other vertebrates permits the translation of research between species. Zebrafish behavior can be studied at both larval and adult stages and recent research has begun to establish zebrafish models for human disease. Fast scan cyclic voltammetry (FSCV) is an electrochemical technique that permits the detection of neurotransmitter release and reuptake. In this study we have used in vitro FSCV to measure the release of analytes in the adult zebrafish telencephalon. We compare different stimulation methods and present a characterization of neurochemical changes in the wild-type zebrafish brain. This study represents the first FSCV recordings in zebrafish, thus paving the way for neurochemical analysis of the fish brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping