PUBLICATION

Diethyl phthalate exposure is associated with embryonic toxicity, fatty liver changes, and hypolipidemia via impairment of lipoprotein functions

Authors
Kim, S.M., Yoo, J.A., Baek, J.M., Cho, K.H.
ID
ZDB-PUB-151002-8
Date
2015
Source
Toxicology in vitro : an international journal published in association with BIBRA   30(1 Pt B): 383-93 (Journal)
Registered Authors
Keywords
atherosclerosis, diethyl phthalate, embryo toxicity, fatty liver, lipoprotein, zebrafish
MeSH Terms
  • Lipoproteins/physiology*
  • Apolipoproteins B/chemistry
  • Animals
  • Fatty Liver/chemically induced*
  • Lipids/blood*
  • Zebrafish/embryology
  • Humans
  • Apolipoprotein A-I/chemistry
  • Embryo, Nonmammalian/drug effects*
  • Phthalic Acids/toxicity*
(all 10)
PubMed
26423653 Full text @ Toxicol. In Vitro
CTD
26423653
Abstract
Diethyl phthalates (DEP) are notorious for their high potential toxicity in endocrinological and reproduction systems in humans and animals. In this study, we investigated the toxic effects of DEP on human lipoproteins, macrophages, and zebrafish embryos. Treatment of human high-density lipoprotein (HDL) with DEP caused oxidation, aggregation, and degradation of lipoproteins. DEP treatment promoted foam cell formation via accelerated phagocytosis of LDL by macrophages as well as exacerbated cellular senescence in human dermal fibroblasts. Injection of DEP (final 5 μM and 10 μM) into zebrafish embryos caused severe embryo death and slower developmental speed. Exposure of zebrafish embryos to water containing DEP (final 11 and 22 ppm) caused early embryonic death along with increased oxidized products and impairment of skeletal development. Adult zebrafish exposed to water containing DEP (final 11 and 22 ppm) for 4 weeks showed severe loss of body weight under both normal diet (ND) and high cholesterol diet (HCD) conditions. ND and HCD groups showed 59% and 49% reduction of plasma total cholesterol (TC), respectively. Serum levels of hepatic inflammation enzymes along with fatty liver changes were significantly elevated by DEP exposure. In conclusion, DEP showed strong pro-atherogenic and pro-senescence effects via severe lipoprotein modification in human cells. DEP caused impairment of embryonic development and severe loss of body weight, hypolipidemia, and fatty liver changes in zebrafish.
Genes / Markers
Figures
No images available
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Kim, S.M., Yoo, J.A., Baek, J.M., Cho, K.H. (2015) Diethyl phthalate exposure is associated with embryonic toxicity, fatty liver changes, and hypolipidemia via impairment of lipoprotein functions. Toxicology in vitro : an international journal published in association with BIBRA. 30(1 Pt B):383-93.