PUBLICATION

Temporal-Spatial Resolution Fate Mapping Reveals Distinct Origins for Embryonic and Adult Microglia in Zebrafish

Authors
Xu, J., Zhu, L., He, S., Wu, Y., Jin, W., Yu, T., Qu, J.Y., Wen, Z.
ID
ZDB-PUB-150930-1
Date
2015
Source
Developmental Cell   34: 632-641 (Journal)
Registered Authors
Wen, Zilong
Keywords
none
MeSH Terms
  • Animals
  • Cell Differentiation*
  • Cell Lineage*
  • Gene Expression Regulation, Developmental*
  • Hematopoiesis/physiology*
  • In Situ Hybridization
  • Mice
  • Microglia/cytology*
  • Microglia/metabolism
  • Neurogenesis/physiology
  • Yolk Sac
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
26418294 Full text @ Dev. Cell
Abstract
Microglia are CNS resident macrophages, and they play important roles in neural development and function. Recent studies have suggested that murine microglia arise from a single source, the yolk sac (YS), yet these studies lack spatial resolution to define the bona fide source(s) for microglia. Here, using light-induced high temporal-spatial resolution fate mapping, we challenge this single-source view by showing that microglia in zebrafish arise from multiple sources. The embryonic/larval microglia originate from the rostral blood island (RBI) region, the equivalent of mouse YS for myelopoiesis, whereas the adult microglia arise from the ventral wall of dorsal aorta (VDA) region, a tissue also producing definitive hematopoiesis in mouse. We further show that the VDA-region-derived microglia are Runx1 dependent, but cMyb independent, and developmentally regulated differently from the RBI region-derived microglia. Our study establishes a new paradigm for investigating the development and function of distinct microglia populations.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes