PUBLICATION
Cystathionine β-synthase regulates endothelial function via protein S-sulfhydration
- Authors
- Saha, S., Chakraborty, P.K., Xiong, X., Dwivedi, S.K., Mustafi, S.B., Leigh, N.R., Ramchandran, R., Mukherjee, P., Bhattacharya, R.
- ID
- ZDB-PUB-150926-2
- Date
- 2016
- Source
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 30(1): 441-56 (Journal)
- Registered Authors
- Leigh, Noah, Ramchandran, Ramani
- Keywords
- VEGFRs, angiogenesis, metabolism, signal transduction
- MeSH Terms
-
- Cell Movement
- Cell Proliferation
- Cystathionine beta-Synthase/genetics
- Cystathionine beta-Synthase/metabolism*
- Endothelium, Vascular/drug effects
- Endothelium, Vascular/metabolism*
- Endothelium, Vascular/physiology
- Glutathione/metabolism
- Human Umbilical Vein Endothelial Cells/drug effects
- Human Umbilical Vein Endothelial Cells/metabolism
- Human Umbilical Vein Endothelial Cells/physiology
- Humans
- Hydrogen Sulfide/metabolism*
- Neuropilins/genetics
- Neuropilins/metabolism
- Second Messenger Systems
- Vascular Endothelial Growth Factor A/pharmacology
- Vascular Endothelial Growth Factor Receptor-2/genetics
- Vascular Endothelial Growth Factor Receptor-2/metabolism
- PubMed
- 26405298 Full text @ FASEB J.
Citation
Saha, S., Chakraborty, P.K., Xiong, X., Dwivedi, S.K., Mustafi, S.B., Leigh, N.R., Ramchandran, R., Mukherjee, P., Bhattacharya, R. (2016) Cystathionine β-synthase regulates endothelial function via protein S-sulfhydration. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 30(1):441-56.
Abstract
Deficiencies of the human cystathionine β-synthase (CBS) enzyme are characterized by a plethora of vascular disorders and hyperhomocysteinemia. However, several clinical trials demonstrated that despite reduction in homocysteine levels, disease outcome remained unaffected, thus the mechanism of endothelial dysfunction is poorly defined. Here, we show that the loss of CBS function in endothelial cells (ECs) leads to a significant down-regulation of cellular hydrogen sulfide (H2S) by 50% and glutathione (GSH) by 40%, respectively. Silencing CBS in ECs compromised phenotypic and signaling responses to the VEGF that were potentiated by decreased transcription of VEGF receptor (VEGFR)-2 and neuropilin (NRP)-1, the primary receptors regulating endothelial function. Transcriptional down-regulation of VEGFR-2 and NRP-1 was mediated by a lack in stability of the transcription factor specificity protein 1 (Sp1), which is a sulfhydration target of H2S at residues Cys68 and Cys755. Reinstating H2S but not GSH in CBS-silenced ECs restored Sp1 levels and its binding to the VEGFR-2 promoter and VEGFR-2, NRP-1 expression, VEGF-dependent proliferation, and migration phenotypes. Thus, our study emphasizes the importance of CBS-mediated protein S-sulfhydration in maintaining vascular health and function.-Saha, S., Chakraborty, P. K., Xiong, X., Dwivedi, S. K. D., Mustafi, S. B., Leigh, N.R., Ramchandran, R., Mukherjee, P., Bhattacharya, R. Cystathionine β-synthase regulates endothelial function via protein S-sulfhydration.
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Mutations / Transgenics
Human Disease / Model
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