ZFIN ID: ZDB-PUB-150924-5
Large-scale live imaging of adult neural stem cells in their endogenous niche
Dray, N., Bedu, S., Vuillemin, N., Alunni, A., Coolen, M., Krecsmarik, M., Supatto, W., Beaurepaire, E., Bally-Cuif, L.
Date: 2015
Source: Development (Cambridge, England)   142(20): 3592-600 (Journal)
Registered Authors: Alunni, Alessandro, Bally-Cuif, Laure, Bedu, Sebastien, Coolen, Marion, Dray, Nicolas
Keywords: SHG/THG, Live imaging, Multiphoton microscopy, Neural stem cell, Pallium, Zebrafish
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Brain/metabolism*
  • Cell Lineage
  • Cell Proliferation
  • Green Fluorescent Proteins/metabolism
  • Hydrogen-Ion Concentration
  • Image Processing, Computer-Assisted/methods
  • Luminescent Proteins/metabolism
  • Microscopy, Fluorescence
  • Neural Stem Cells/cytology*
  • Neuroimaging/methods*
  • Stem Cell Niche*
  • Telencephalon
  • Temperature
  • Transgenes
  • Zebrafish
PubMed: 26395477 Full text @ Development
Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of more than 1,000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.