PUBLICATION
MK-801 increases locomotor activity in a context-dependent manner in zebrafish
- Authors
- Tran, S., Muraleetharan, A., Fulcher, N., Chatterjee, D., Gerlai, R.
- ID
- ZDB-PUB-150901-10
- Date
- 2016
- Source
- Behavioural brain research 296: 26-9 (Other)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- Locomotor activity, MK-801, dopamine, zebrafish
- MeSH Terms
-
- 3,4-Dihydroxyphenylacetic Acid/metabolism*
- Animals
- Behavior, Animal/drug effects*
- Brain/drug effects*
- Dizocilpine Maleate/administration & dosage
- Dizocilpine Maleate/pharmacology*
- Dopamine/metabolism*
- Excitatory Amino Acid Antagonists/administration & dosage
- Excitatory Amino Acid Antagonists/pharmacology*
- Motor Activity/drug effects*
- Zebrafish/metabolism*
- PubMed
- 26318934 Full text @ Behav. Brain Res.
Citation
Tran, S., Muraleetharan, A., Fulcher, N., Chatterjee, D., Gerlai, R. (2016) MK-801 increases locomotor activity in a context-dependent manner in zebrafish. Behavioural brain research. 296:26-9.
Abstract
Zebrafish have become a popular animal model for behavioral neuroscience with an increasing number of studies examining the effects of pharmacological compounds targeting the brain. Exposure to MK-801, a non-competitive N-methyl-D-aspartate receptor antagonist has been shown to increase locomotor activity in zebrafish. However, others have failed to replicate this finding as several contradicting studies report no changes in locomotor activity following exposure to similar doses. In the current study we reconcile these behavioral reports by demonstrating that zebrafish do not exhibit changes in locomotor activity during exposure to non-sedative doses of MK-801. Interestingly, zebrafish do exhibit significant increases in locomotion if pre-treated with MK-801 followed by subsequent testing in a novel environment, which suggests the effects of MK-801 are context-dependent. In addition, we examine the potential role of the dopaminergic system in mediating MK-801's locomotor stimulant effect by quantifying the levels of dopamine and its metabolite 3,4-dihydroxyphenylacetic acid (DOPAC) in the brains of zebrafish following a 30minute exposure to 10μM of MK-801 (the dose found to induce the largest increase in locomotor activity). Our findings indicate that the MK-801-induced increase in locomotor activity is not accompanied by changes in whole-brain levels of dopamine or DOPAC. Overall, our results suggest that MK-801's context-dependent locomotor stimulant effect may be independent of whole-brain dopaminergic activation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping