PUBLICATION
Elevated dopamine concentration in light-adapted zebrafish retinas is correlated with increased dopamine synthesis and metabolism
- Authors
- Connaughton, V.P., Wetzell, B.B., Arneson, L.S., DeLucia, V., Riley, A.L.
- ID
- ZDB-PUB-150728-4
- Date
- 2015
- Source
- Journal of neurochemistry 135(1): 101-8 (Journal)
- Registered Authors
- Connaughton, Victoria P.
- Keywords
- HPLC, Dopamine, adaptational state, interplexiform cells, retina, tyrosine hydroxylase
- MeSH Terms
-
- 3,4-Dihydroxyphenylacetic Acid/metabolism
- Adaptation, Physiological
- Animals
- Circadian Rhythm/physiology*
- Dopamine/metabolism*
- Light*
- Photoperiod
- Retina/metabolism*
- Tyrosine 3-Monooxygenase/metabolism*
- Zebrafish
- PubMed
- 26212704 Full text @ J. Neurochem.
Citation
Connaughton, V.P., Wetzell, B.B., Arneson, L.S., DeLucia, V., Riley, A.L. (2015) Elevated dopamine concentration in light-adapted zebrafish retinas is correlated with increased dopamine synthesis and metabolism. Journal of neurochemistry. 135(1):101-8.
Abstract
Probing zebrafish (Danio rerio) retinal cryostat sections, collected either 8 hr into the light or dark cycle, with an antibody to tyrosine hydroxylase (TH) identified a single population of immunopositive cells in the inner retina. However, the observed labeling patterns were not identical in both sets of tissues - label intensity was brighter in light-adapted tissue. This difference was quantified by probing Western Blots of retinal homogenates with the same TH antibody, which showed that TH expression is increased 42% in light-adapted tissue. High performance liquid chromatography with electrochemical detection revealed that the concentrations of both dopamine and DOPAC (3,4-dihydroxyphenylacetic acid) are also elevated in light-adapted zebrafish retinal tissue. Dopamine levels increased by 14% and DOPAC levels increased by 25% when measured in retinal homogenates harvested during the light cycle. These results indicate that dopamine levels in zebrafish retina are significantly increased in light-adapted tissue. The increase in dopamine content is correlated with an increase in both TH and DOPAC, suggesting that changes in dopamine concentration are due to light-adaptive changes in the synthesis, release and metabolism of dopamine. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping