PUBLICATION

Remodeling of Mitochondrial Flashes in Muscular Development and Dystrophy in Zebrafish

Authors
Zhang, M., Sun, T., Jian, C., Lei, L., Han, P., Lv, Q., Yang, R., Zhou, X., Xu, J., Hu, Y., Men, Y., Huang, Y., Zhang, C., Zhu, X., Wang, X., Cheng, H., Xiong, J.W.
ID
ZDB-PUB-150718-1
Date
2015
Source
PLoS One   10: e0132567 (Journal)
Registered Authors
Han, Peidong, Xiong, Jing-Wei
Keywords
Mitochondria, Skeletal muscles, Embryos, Zebrafish, Skeletal muscle fibers, Dystrophin, Muscle fibers, Muscle cells
MeSH Terms
  • Zebrafish
  • Membrane Proteins/antagonists & inhibitors
  • Membrane Proteins/genetics*
  • Membrane Proteins/metabolism
  • Animals
  • Actins/genetics
  • Actins/metabolism
  • Bacterial Proteins/genetics
  • Bacterial Proteins/metabolism
  • Morpholinos/genetics
  • Morpholinos/metabolism
  • Muscle, Skeletal/metabolism*
  • Muscle, Skeletal/pathology
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Mitochondrial Dynamics/genetics*
  • Superoxide Dismutase/genetics
  • Superoxide Dismutase/metabolism
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Muscle Proteins/antagonists & inhibitors
  • Muscle Proteins/genetics*
  • Muscle Proteins/metabolism
  • NADP/metabolism
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Animals, Genetically Modified
  • Mitochondria/metabolism*
  • Mitochondria/pathology
  • Muscle Development/genetics
  • Gene Expression Regulation, Developmental
  • Embryo, Nonmammalian
  • Genes, Reporter
  • Disease Models, Animal
  • Muscular Dystrophies/genetics*
  • Muscular Dystrophies/metabolism
  • Muscular Dystrophies/pathology
  • Time-Lapse Imaging
  • Signal Transduction
(all 40)
PubMed
26186000 Full text @ PLoS One
Abstract
Mitochondrial flash (mitoflash) is a highly-conserved, universal, and physiological mitochondrial activity in isolated mitochondria, intact cells, and live organisms. Here we investigated developmental and disease-related remodeling of mitoflash activity in zebrafish skeletal muscles. In transgenic zebrafish expressing the mitoflash reporter cpYFP, in vivo imaging revealed that mitoflash frequency and unitary properties underwent multiphasic and muscle type-specific changes, accompanying mitochondrial morphogenesis from 2 to 14 dpf. In particular, short (S)-type mitoflashes predominated in early muscle formation, then S-, transitory (T)- and regular (R)-type mitoflashes coexisted during muscle maturation, followed by a switch to R-type mitoflashes in mature skeletal muscles. In early development of muscular dystrophy, we found accelerated S- to R-type mitoflash transition and reduced mitochondrial NAD(P)H amidst a remarkable cell-to-cell heterogeneity. This study not only unravels a profound functional and morphological remodeling of mitochondria in developing and diseased skeletal muscles, but also underscores mitoflashes as a useful reporter of mitochondrial function in milieu of live animals under physiological and pathophysiological conditions.
Genes / Markers
Figures
Figure Gallery (9 images)
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
pku341TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    dmdMO2-dmdMRPHLNO
    1 - 1 of 1
    Show
    Fish
    Antibodies
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    cpYFPEFGcpYFP
    1 - 1 of 1
    Show
    Mapping
    No data available
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    Citation
    Zhang, M., Sun, T., Jian, C., Lei, L., Han, P., Lv, Q., Yang, R., Zhou, X., Xu, J., Hu, Y., Men, Y., Huang, Y., Zhang, C., Zhu, X., Wang, X., Cheng, H., Xiong, J.W. (2015) Remodeling of Mitochondrial Flashes in Muscular Development and Dystrophy in Zebrafish. PLoS One. 10:e0132567.