PUBLICATION
Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration
- Authors
- Trivedi, V., Truong, T.V., Trinh, l.e. .A., Holland, D.B., Liebling, M., Fraser, S.E.
- ID
- ZDB-PUB-150627-2
- Date
- 2015
- Source
- Biomedical Optics Express 6: 2056-66 (Journal)
- Registered Authors
- Liebling, Michael, Trinh, Le
- Keywords
- (170.1420) Biology, (170.3010) Image reconstruction techniques, (180.0180) Microscopy, (190.0190) Nonlinear optics
- MeSH Terms
- none
- PubMed
- 26114028 Full text @ Biomed. Opt. Express
Citation
Trivedi, V., Truong, T.V., Trinh, l.e. .A., Holland, D.B., Liebling, M., Fraser, S.E. (2015) Dynamic structure and protein expression of the live embryonic heart captured by 2-photon light sheet microscopy and retrospective registration. Biomedical Optics Express. 6:2056-66.
Abstract
We present an imaging and image reconstruction pipeline that captures the dynamic three-dimensional beating motion of the live embryonic zebrafish heart at subcellular resolution. Live, intact zebrafish embryos were imaged using 2-photon light sheet microscopy, which offers deep and fast imaging at 70 frames per second, and the individual optical sections were assembled into a full 4D reconstruction of the beating heart using an optimized retrospective image registration algorithm. This imaging and reconstruction platform permitted us to visualize protein expression patterns at endogenous concentrations in zebrafish gene trap lines.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping