|ZFIN ID: ZDB-PUB-150626-7|
A high-throughput assay for quantifying appetite and digestive dynamics
Jordi, J., Guggiana-Nilo, D., Soucy, E., Song, E.Y., Wee, C.L., Engert, F.
|Source:||American journal of physiology. Regulatory, integrative and comparative physiology 309(4): R345-57 (Journal)|
|Registered Authors:||Engert, Florian|
|Keywords:||DiR' dye, appetite, hunger, satiation, zebrafish|
|PubMed:||26108871 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.|
Jordi, J., Guggiana-Nilo, D., Soucy, E., Song, E.Y., Wee, C.L., Engert, F. (2015) A high-throughput assay for quantifying appetite and digestive dynamics. American journal of physiology. Regulatory, integrative and comparative physiology. 309(4):R345-57.
ABSTRACTFood intake and digestion are vital functions and their dysregulation is fundamental for many human diseases. Current methods do not support their dynamic quantification on large scales in unrestrained vertebrates. Here we combine an infrared macroscope with fluorescently labeled food to quantify feeding behavior and intestinal nutrient metabolism with high temporal resolution, sensitivity and throughput in naturally behaving zebrafish larvae. Using this method and rate-based modeling, we demonstrate that zebrafish larvae match nutrient intake to their bodily demand and that larvae adjust their digestion rate according to the ingested meal size. Such adaptive feedback mechanisms make this model system amenable to identify potential chemical modulators. As proof of concept we demonstrate that nicotine, L-lysine, ghrelin and insulin have analogous impact on food intake as in mammals. Consequently, the method presented here will promote large-scale translational research of food intake and digestive function in a naturally behaving vertebrate.