PUBLICATION
Mitotic cell rounding and epithelial thinning regulate lumen growth and shape
- Authors
- Hoijman, E., Rubbini, D., Colombelli, J., Alsina, B.
- ID
- ZDB-PUB-150617-6
- Date
- 2015
- Source
- Nature communications 6: 7355 (Journal)
- Registered Authors
- Alsina, Berta
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Shape*
- Ear, Inner/cytology
- Ear, Inner/embryology*
- Embryo, Nonmammalian
- Epithelial Cells/cytology*
- Imaging, Three-Dimensional
- Intracellular Fluid/metabolism
- Mitosis*
- Organogenesis
- Zebrafish
- PubMed
- 26077034 Full text @ Nat. Commun.
Citation
Hoijman, E., Rubbini, D., Colombelli, J., Alsina, B. (2015) Mitotic cell rounding and epithelial thinning regulate lumen growth and shape. Nature communications. 6:7355.
Abstract
Many organ functions rely on epithelial cavities with particular shapes. Morphogenetic anomalies in these cavities lead to kidney, brain or inner ear diseases. Despite their relevance, the mechanisms regulating lumen dimensions are poorly understood. Here, we perform live imaging of zebrafish inner ear development and quantitatively analyse the dynamics of lumen growth in 3D. Using genetic, chemical and mechanical interferences, we identify two new morphogenetic mechanisms underlying anisotropic lumen growth. The first mechanism involves thinning of the epithelium as the cells change their shape and lose fluids in concert with expansion of the cavity, suggesting an intra-organ fluid redistribution process. In the second mechanism, revealed by laser microsurgery experiments, mitotic rounding cells apicobasally contract the epithelium and mechanically contribute to expansion of the lumen. Since these mechanisms are axis specific, they not only regulate lumen growth but also the shape of the cavity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping