PUBLICATION

Vascular Mural Cells Promote Noradrenergic Differentiation of Embryonic Sympathetic Neurons

Authors
Fortuna, V., Pardanaud, L., Brunet, I., Ola, R., Ristori, E., Santoro, M.M., Nicoli, S., Eichmann, A.
ID
ZDB-PUB-150616-4
Date
2015
Source
Cell Reports   11(11): 1786-96 (Journal)
Registered Authors
Nicoli, Stefania, Ristori, Emma, Santoro, Massimo
Keywords
none
MeSH Terms
  • Receptors, Platelet-Derived Growth Factor/genetics
  • Receptors, Platelet-Derived Growth Factor/metabolism
  • Neurogenesis*
  • Aorta/cytology
  • Aorta/embryology
  • Endothelial Cells/cytology*
  • Endothelial Cells/metabolism
  • Endothelium, Vascular/cytology*
  • Endothelium, Vascular/embryology
  • Animals
  • Adrenergic Neurons/cytology*
  • Adrenergic Neurons/metabolism
  • Sympathetic Fibers, Postganglionic/cytology*
  • Sympathetic Fibers, Postganglionic/embryology
  • Zebrafish
  • Neural Stem Cells/cytology*
  • Neural Stem Cells/metabolism
(all 17)
PubMed
26074079 Full text @ Cell Rep.
Abstract
The sympathetic nervous system controls smooth muscle tone and heart rate in the cardiovascular system. Postganglionic sympathetic neurons (SNs) develop in close proximity to the dorsal aorta (DA) and innervate visceral smooth muscle targets. Here, we use the zebrafish embryo to ask whether the DA is required for SN development. We show that noradrenergic (NA) differentiation of SN precursors temporally coincides with vascular mural cell (VMC) recruitment to the DA and vascular maturation. Blocking vascular maturation inhibits VMC recruitment and blocks NA differentiation of SN precursors. Inhibition of platelet-derived growth factor receptor (PDGFR) signaling prevents VMC differentiation and also blocks NA differentiation of SN precursors. NA differentiation is normal in cloche mutants that are devoid of endothelial cells but have VMCs. Thus, PDGFR-mediated mural cell recruitment mediates neurovascular interactions between the aorta and sympathetic precursors and promotes their noradrenergic differentiation.
Genes / Markers
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
la116TgTransgenic Insertion
    s5
      		Point Mutation
      s916TgTransgenic Insertion
        zdf15TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          tnnt2aMO1-tnnt2aMRPHLNO
          1 - 1 of 1
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          Fish
          No data available
          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          GFPEFGGFP
          mCherryEFGmCherry
          1 - 3 of 3
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          Mapping
          No data available
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          Citation
          Fortuna, V., Pardanaud, L., Brunet, I., Ola, R., Ristori, E., Santoro, M.M., Nicoli, S., Eichmann, A. (2015) Vascular Mural Cells Promote Noradrenergic Differentiation of Embryonic Sympathetic Neurons. Cell Reports. 11(11):1786-96.