PUBLICATION
Vascular Mural Cells Promote Noradrenergic Differentiation of Embryonic Sympathetic Neurons
- Authors
- Fortuna, V., Pardanaud, L., Brunet, I., Ola, R., Ristori, E., Santoro, M.M., Nicoli, S., Eichmann, A.
- ID
- ZDB-PUB-150616-4
- Date
- 2015
- Source
- Cell Reports 11(11): 1786-96 (Journal)
- Registered Authors
- Nicoli, Stefania, Ristori, Emma, Santoro, Massimo
- Keywords
- none
- MeSH Terms
-
- Adrenergic Neurons/cytology*
- Adrenergic Neurons/metabolism
- Neurogenesis*
- Receptors, Platelet-Derived Growth Factor/genetics
- Receptors, Platelet-Derived Growth Factor/metabolism
- Aorta/cytology
- Aorta/embryology
- Animals
- Endothelium, Vascular/cytology*
- Endothelium, Vascular/embryology
- Sympathetic Fibers, Postganglionic/cytology*
- Sympathetic Fibers, Postganglionic/embryology
- Zebrafish
- Neural Stem Cells/cytology*
- Neural Stem Cells/metabolism
- Endothelial Cells/cytology*
- Endothelial Cells/metabolism
- PubMed
- 26074079 Full text @ Cell Rep.
Citation
Fortuna, V., Pardanaud, L., Brunet, I., Ola, R., Ristori, E., Santoro, M.M., Nicoli, S., Eichmann, A. (2015) Vascular Mural Cells Promote Noradrenergic Differentiation of Embryonic Sympathetic Neurons. Cell Reports. 11(11):1786-96.
Abstract
The sympathetic nervous system controls smooth muscle tone and heart rate in the cardiovascular system. Postganglionic sympathetic neurons (SNs) develop in close proximity to the dorsal aorta (DA) and innervate visceral smooth muscle targets. Here, we use the zebrafish embryo to ask whether the DA is required for SN development. We show that noradrenergic (NA) differentiation of SN precursors temporally coincides with vascular mural cell (VMC) recruitment to the DA and vascular maturation. Blocking vascular maturation inhibits VMC recruitment and blocks NA differentiation of SN precursors. Inhibition of platelet-derived growth factor receptor (PDGFR) signaling prevents VMC differentiation and also blocks NA differentiation of SN precursors. NA differentiation is normal in cloche mutants that are devoid of endothelial cells but have VMCs. Thus, PDGFR-mediated mural cell recruitment mediates neurovascular interactions between the aorta and sympathetic precursors and promotes their noradrenergic differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping