PUBLICATION

Defining the phenotype of neutrophils following reverse migration in zebrafish

Authors
Ellett, F., Elks, P.M., Robertson, A.L., Ogryzko, N.V., Renshaw, S.A.
ID
ZDB-PUB-150614-1
Date
2015
Source
Journal of Leukocyte Biology   98(6): 975-81 (Journal)
Registered Authors
Elks, Phil, Ellett, Felix, Ogryzko, Nikolay, Renshaw, Steve A., Robertson, Anne
Keywords
chemotaxis, granulocytes, infection, inflammation, phagocytosis
MeSH Terms
  • Animals
  • Animals, Genetically Modified/genetics
  • Animals, Genetically Modified/immunology
  • Cell Movement/genetics
  • Cell Movement/immunology*
  • Inflammation/genetics
  • Inflammation/immunology
  • Neutrophils/immunology*
  • Staphylococcus aureus/immunology
  • Zebrafish/genetics
  • Zebrafish/immunology*
PubMed
26071567 Full text @ J. Leukoc. Biol.
Abstract
Stimulation of neutrophil reverse migration presents an attractive, alternative therapeutic pathway to driving inflammation resolution. However, little is known about whether the activity of wound-experienced neutrophils is altered and whether encouraging dispersal of such neutrophils back into the body may have undesirable consequences. This study used a zebrafish tail transection inflammation model, in combination with a photoconvertible neutrophil transgenic line, to allow internally controlled, simultaneous comparison of reverse-migrated neutrophils with naïve neutrophils in the presence and absence of secondary insult. Detailed microscopy revealed that reverse-migrated neutrophils exhibited an activated morphology but responded normally to secondary insult and are able to mount an effective antimicrobial response to Staphylococcus aureus. These results support a model in which reverse-migrated neutrophils exhibit no long-term behavioral alterations and encourage the notion of enhanced reverse migration as a viable target for pharmaceutical manipulation.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes