PUBLICATION
Synergistic potentials of coffee on injured pancreatic islets and insulin action via KATP channel-blocking in zebrafish
- Authors
- Nam, Y.H., Hong, B.N., Rodriguez, M.I., Ji, M.G., Kim, K., Kim, U.J., Kang, T.H.
- ID
- ZDB-PUB-150521-3
- Date
- 2015
- Source
- Journal of Agricultural and Food Chemistry 63(23): 5612-21 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Alkaloids/pharmacology
- Alloxan/adverse effects
- Animals
- Caffeine/pharmacology
- Chlorogenic Acid/pharmacology
- Coffea/chemistry*
- Coffee/chemistry*
- Drug Synergism
- Insulin/metabolism*
- Islets of Langerhans/drug effects*
- Islets of Langerhans/injuries
- Islets of Langerhans/metabolism
- KATP Channels/antagonists & inhibitors*
- KATP Channels/metabolism
- Plant Extracts/pharmacology*
- Zebrafish
- PubMed
- 25993315 Full text @ J. Agric. Food Chem.
Citation
Nam, Y.H., Hong, B.N., Rodriguez, M.I., Ji, M.G., Kim, K., Kim, U.J., Kang, T.H. (2015) Synergistic potentials of coffee on injured pancreatic islets and insulin action via KATP channel-blocking in zebrafish. Journal of Agricultural and Food Chemistry. 63(23):5612-21.
Abstract
Pancreatic islets (PIs) are damaged under diabetic conditions, which can cause decreases in PI size. In this study, we examined the regenerative effects of coffee and its components (caffeine, CFI; trigonelline, TRG; chlorogenic acid, CGA) on zebrafish larval PIs and β-cells damaged via alloxan (AX) administration. In addition, we investigated the influence of coffee and its active components on KATP channels using diazoxide (DZ) as a KATP channel activator. PI size and fluorescence intensity were significantly increased in the coffee-treated group relative to the no-treatment group (P<0.0001). Coffee exerted significant regenerative effects on pancreatic β-cells (P=0.006). Furthermore, treatment with TRG and CGA resulted in recovery from PI damage, and the combination of TRG/CGA had a synergistic effect. In conclusion, our results show that coffee can exert beneficial effects on PIs damaged by AX, and furthermore that coffee has the potential to be used as a blocker of pancreatic β-cell K+ channels.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping