PUBLICATION
Antioxidant activity of polysaccharide purified from Acanthopanax koreanum Nakai stems in vitro and in vivo zebrafish model
- Authors
- Kang, M.C., Kim, S.Y., Kim, E.A., Lee, J.H., Kim, Y.S., Yu, S.K., Chae, J.B., Choe, I.H., Cho, J.H., Jeon, Y.J.
- ID
- ZDB-PUB-150513-3
- Date
- 2015
- Source
- Carbohydrate Polymers 127: 38-46 (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- Acanthopanax koreanum Nakai, Antioxidant activity, Enzyme-assistant extract, Oxidative stress, Polysaccharide, Zebrafish
- MeSH Terms
-
- Animals
- Antioxidants/chemistry
- Antioxidants/isolation & purification
- Antioxidants/pharmacology*
- Araliaceae/chemistry*
- Cell Death/drug effects
- Cells, Cultured
- Chlorocebus aethiops
- Free Radicals/antagonists & inhibitors
- Hydrogen Peroxide/antagonists & inhibitors
- Hydrogen Peroxide/pharmacology
- Models, Animal
- Oxidative Stress/drug effects
- Plant Stems/chemistry*
- Polysaccharides/chemistry
- Polysaccharides/isolation & purification*
- Polysaccharides/pharmacology*
- Reactive Oxygen Species/metabolism
- Vero Cells
- Zebrafish*
- PubMed
- 25965454 Full text @ Carbohydr. Polym.
Citation
Kang, M.C., Kim, S.Y., Kim, E.A., Lee, J.H., Kim, Y.S., Yu, S.K., Chae, J.B., Choe, I.H., Cho, J.H., Jeon, Y.J. (2015) Antioxidant activity of polysaccharide purified from Acanthopanax koreanum Nakai stems in vitro and in vivo zebrafish model. Carbohydrate Polymers. 127:38-46.
Abstract
In this study, the antioxidative effects of a purified polysaccharide isolated from the stems of Acanthopanax koreanum Nakai (ASP) on hydrogen peroxide-induced oxidative stress was investigated both in vitro and in vivo using a zebrafish model. A. koreanum Nakai stem was hydrolyzed using five carbohydrases and five proteases for the enzyme-assistant extraction. Of the enzyme-assistant extracts, the Protamex extract exhibited the highest yield and a potent scavenging activity against free radicals. Ethanol-added separation and anion exchange chromatography were conducted to identify the active polysaccharide. The purified polysaccharide significantly scavenged hydrogen peroxide and reduced hydrogen peroxide-induced cell death in Vero cells and in zebrafish. The results reveal that ASP is a useful antioxidant polysaccharide and might be available for relevant industrial applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping