PUBLICATION
Abstracting the principles of development using imaging and modeling
- Authors
- Xiong, F., Megason, S.G.
- ID
- ZDB-PUB-150508-11
- Date
- 2015
- Source
- Integrative biology : quantitative biosciences from nano to macro 7(6): 633-42 (Other)
- Registered Authors
- Megason, Sean, Xiong, Fengzhu
- Keywords
- none
- MeSH Terms
-
- Animals
- Body Patterning
- Developmental Biology/methods*
- Developmental Biology/trends
- Models, Biological*
- Optical Imaging/methods
- Systems Biology
- Zebrafish/embryology
- PubMed
- 25946995 Full text @ Integr. Biol. (Camb)
Citation
Xiong, F., Megason, S.G. (2015) Abstracting the principles of development using imaging and modeling. Integrative biology : quantitative biosciences from nano to macro. 7(6):633-42.
Abstract
Here we look at modern developmental biology with a focus on the relationship between different approaches of investigation. We argue that direct imaging is a powerful approach not only for obtaining descriptive information but also for model generation and testing that lead to mechanistic insights. Modeling, on the other hand, conceptualizes imaging data and provides guidance to perturbations. The inquiry progresses most efficiently when a trinity of approaches-quantitative imaging (measurement), modeling (theory) and perturbation (test)-are pursued in concert, but not when one approach is dominant. Using recent studies of the zebrafish system, we show how this combination has effectively advanced classic topics in developmental biology compared to a perturbation-centric approach. Finally, we show that interdisciplinary expertise and perhaps specialization are necessary for carrying out a systematic approach, and discuss the technical hurdles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping