PUBLICATION
A fast and selective two-photon phosphorescent probe for the imaging of nitric oxide in mitochondria
- Authors
- Chen, X., Sun, L., Chen, Y., Cheng, X., Wu, W., Ji, L., Chao, H.
- ID
- ZDB-PUB-150506-3
- Date
- 2015
- Source
- Biomaterials 58: 72-81 (Journal)
- Registered Authors
- Chen, Yu
- Keywords
- Iridium complex, Mitochondria, Nitric oxide, Two-photon probe, Zebrafish
- MeSH Terms
-
- Animals
- Cell Adhesion
- Diamines/chemistry
- HeLa Cells
- Humans
- Iridium/chemistry
- Luminescence
- Mass Spectrometry
- Microscopy, Confocal/instrumentation*
- Mitochondria/metabolism*
- Molecular Probes/chemistry*
- Nitric Oxide/chemistry*
- Phenanthrolines/chemistry
- Photons
- Spheroids, Cellular
- Tumor Cells, Cultured
- Zebrafish
- PubMed
- 25941784 Full text @ Biomaterials
Citation
Chen, X., Sun, L., Chen, Y., Cheng, X., Wu, W., Ji, L., Chao, H. (2015) A fast and selective two-photon phosphorescent probe for the imaging of nitric oxide in mitochondria. Biomaterials. 58:72-81.
Abstract
Nitric oxide (NO) is a vitally important cellular messenger molecule related to numerous physiological events and diseases. It is more than 10 years now that mitochondria are suspected to be sources of nitric oxide. Hence, mitochondrial NO tracking probes play an indispensable role in NO behavior analysis. However, a majority of previously reported NO probes can only be employed under one-photon microscopy, often with several drawbacks during application. In the present study, an iridium(III) complex containing 1,10-phenanthroline-5,6-diamine (Ir-Mito-NO) was synthesized and determined to possess high specificity to NO in mitochondria, low cytotoxicity, and rapid and specific "off-on" two-photon phosphorescence. Thus, this complex was developed to image mitochondrial NO levels in living cells, 3D multicellular spheroids and zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping