PUBLICATION

Control of Wnt5b secretion by wntless modulates chondrogenic cell proliferation through fine-tuning fgf3 expression

Authors

Wu, B.T., Wen, S.H., Hwang, S.P., Huang, C.J., Kuan, Y.S.

ID

ZDB-PUB-150503-1

Date

2015

Source

Journal of Cell Science 128(12): 2328-39 (Journal)

Registered Authors

Keywords

none

MeSH Terms

Chondrogenesis/physiology*
Morpholinos/pharmacology
Immunoenzyme Techniques
Animals
Apoptosis
In Situ Hybridization
Cell Proliferation*
Wnt Proteins/metabolism*
Fibroblast Growth Factor 3/metabolism*
Gene Expression Regulation, Developmental*
Wnt-5a Protein
Cells, Cultured
Embryonic Development
Embryo, Nonmammalian/cytology
Embryo, Nonmammalian/metabolism
Receptors, G-Protein-Coupled/metabolism*
Cartilage/growth & development*
Cartilage/metabolism
Zebrafish/embryology*
Zebrafish/metabolism
Zebrafish Proteins/metabolism*

(all 21)

PubMed

25934698 Full text @ J. Cell Sci.

Abstract

Wnts and Fgfs regulate various tissues development in vertebrates. However, how regional Wnt or Fgf activities are established and how they interact in any given developmental event is elusive. We have investigated the Wnt-mediated craniofacial cartilage development in zebrafish and found that fgf3 expression in the pharyngeal pouches is differentially reduced along the anteroposterior axis in wnt5b mutants and wntless (wls) morphants, but its expression is normal in wnt9a and wnt11 morphants. Introducing fgf3 mRNAs rescued the cartilage defects in Wnt5b and Wls-deficient larvae. In wls morphants, endogenous Wls expression is not detectable but maternally deposited Wls is present in eggs. That might account for the lack of axis defects in wls morphants. Secretion of endogenous Wnt5b but not Wnt11 was affected in the pharyngeal of Wls morphants, indicating that Wls is not involved in every Wnt secretion events. Furthermore, cell proliferation but not apoptosis in the developing jaw was affected in Wnt5b and Wls-deficient embryos. Therefore, Wnt5b requires Wls for secretion and regulates the proliferation of chondrogenic cells through fine-tuning the expression of fgf3 during jaw cartilage development.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-wls	standard conditions	Prim-5	neural crest cell migration process quality, normal	Fig. 5
AB + MO1-wls	standard conditions	Prim-5	pharyngeal arch apoptotic process occurrence, normal	Fig. 6
AB + MO1-wls	standard conditions	Prim-5	pharyngeal arch cell population proliferation occurrence, normal	Fig. 6
AB + MO1-wls	standard conditions	Long-pec	embryonic viscerocranium morphogenesis decreased process quality, abnormal	Fig. 3
AB + MO1-wls	standard conditions	Long-pec	pharyngeal arch apoptotic process occurrence, normal	Fig. 7
AB + MO1-wls	standard conditions	Long-pec	pharyngeal arch cartilage development decreased process quality, abnormal	Fig. 7
AB + MO1-wls	standard conditions	Long-pec	pharyngeal arch cell population proliferation decreased occurrence, abnormal	Fig. 7
AB + MO1-wls	standard conditions	Long-pec	pharyngeal arch Wnt protein secretion decreased occurrence, abnormal	Fig. 4
AB + MO1-wls	standard conditions	Long-pec	pharyngeal pouch 1 decreased object quality, abnormal	Fig. 3
AB + MO1-wls	standard conditions	Long-pec	pharyngeal pouch 2 decreased object quality, abnormal	Fig. 3

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
ti265		Point Mutation	wnt5b

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
wls	MO1-wls	MRPHLNO
wnt9a	MO1-wnt9a	MRPHLNO
wnt11f2	MO1-wnt11f2	MRPHLNO

Fish

Fish
AB
AB + MO1-wls
AB + MO1-wnt9a
AB + MO1-wnt11f2
wnt5b^ti265/ti265
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Wu et al., 2015 ZDB-PUB-150503-1 PMID:25934698

Control of Wnt5b secretion by wntless modulates chondrogenic cell proliferation through fine-tuning fgf3 expression

Wu et al., 2015

ZDB-PUB-150503-1
PMID:25934698