PUBLICATION
Maternal and Zygotic Sphingosine Kinase 2 are Indispensable for Cardiac Development in Zebrafish
- Authors
- Hisano, Y., Inoue, A., Okudaira, M., Taimatsu, K., Matsumoto, H., Kotani, H., Ohga, R., Aoki, J., Kawahara, A.
- ID
- ZDB-PUB-150425-7
- Date
- 2015
- Source
- The Journal of biological chemistry 290(24): 14841-51 (Journal)
- Registered Authors
- Kawahara, Atsuo
- Keywords
- lipid metabolism, lipid signaling, lipid synthesis, lipid transport, sphingolipid
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Female
- Heart/embryology*
- Isoenzymes/chemistry
- Isoenzymes/genetics
- Isoenzymes/metabolism*
- Molecular Sequence Data
- Mutation
- Phosphotransferases (Alcohol Group Acceptor)/chemistry
- Phosphotransferases (Alcohol Group Acceptor)/genetics
- Phosphotransferases (Alcohol Group Acceptor)/metabolism*
- Pregnancy
- Sequence Homology, Amino Acid
- Zebrafish
- Zygote/enzymology*
- PubMed
- 25907554 Full text @ J. Biol. Chem.
Citation
Hisano, Y., Inoue, A., Okudaira, M., Taimatsu, K., Matsumoto, H., Kotani, H., Ohga, R., Aoki, J., Kawahara, A. (2015) Maternal and Zygotic Sphingosine Kinase 2 are Indispensable for Cardiac Development in Zebrafish. The Journal of biological chemistry. 290(24):14841-51.
Abstract
Sphingosine-1-phosphate (S1P) is synthesized from sphingosine by sphingosine kinases (SPHK1 and SPHK2) in invertebrates and vertebrates, whereas specific receptors for S1P (S1PRs) selectively appear in vertebrates, suggesting that S1P acquires novel functions in vertebrates. Because the developmental functions of SPHK1 and SPHK2 remain obscure in vertebrates, we generated sphk1 or sphk2 gene-disrupted zebrafish by introducing premature stop codons in their coding regions using transcription activator-like effector nucleases. Both zygotic sphk1 and sphk2 zebrafish mutants exhibited no obvious developmental defects and grew to adults. The maternal-zygotic sphk2 mutant (MZsphk2), but not the maternal-zygotic sphk1 mutant and maternal sphk2 mutant, had a defect in the cardiac progenitor migration and a concomitant decrease in S1P level, leading to a two-heart phenotype (cardia bifida). Cardia bifida in MZsphk2, which was rescued by injecting sphk2 mRNA, was a phenotype identical to that of zygotic mutants of the S1P transporter spns2 and S1P receptor s1pr2, indicating that the Sphk2-Spns2-S1pr2 axis regulates the cardiac progenitor migration in zebrafish. The requirement for maternal-zygotic Sphk2 presents the contribution of maternally supplied lipid mediators during vertebrate organogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping