PUBLICATION
Kisspeptin1 modulates odorant-evoked fear response via two serotonin receptor subtypes (5-HT1A and 5-HT2) in zebrafish
- Authors
- Nathan, F.M., Ogawa, S., Parhar, I.S.
- ID
- ZDB-PUB-150331-9
- Date
- 2015
- Source
- Journal of neurochemistry 133(6): 870-8 (Journal)
- Registered Authors
- Ogawa, Satoshi
- Keywords
- Methysergide, Raphe, WAY 100635, geriseum central, olfactory
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects
- Behavior, Animal/physiology*
- Fear/physiology*
- Kisspeptins/metabolism*
- Male
- Receptor, Serotonin, 5-HT1A/metabolism*
- Receptors, Serotonin, 5-HT2/metabolism*
- Serotonin 5-HT1 Receptor Antagonists/pharmacology
- Serotonin 5-HT2 Receptor Antagonists/pharmacology
- Zebrafish
- Zebrafish Proteins/metabolism*
- PubMed
- 25818845 Full text @ J. Neurochem.
Citation
Nathan, F.M., Ogawa, S., Parhar, I.S. (2015) Kisspeptin1 modulates odorant-evoked fear response via two serotonin receptor subtypes (5-HT1A and 5-HT2) in zebrafish. Journal of neurochemistry. 133(6):870-8.
Abstract
Kiss1, a neuropeptide predominantly expressed in the habenula, modulates the serotonin (5-HT) system to decrease odorant cue (alarm substance, AS)-evoked fear behaviour in the zebrafish. The purpose of this study was to assess the interaction of Kiss1 with the 5-HT system as well as to determine the involvement of the 5-HT receptor subtypes in AS-evoked fear. We utilised 0. 28 mg/kg WAY100635 (WAY), a selective 5-HT1A receptor antagonist, to observe the effects of Kiss1 administration on AS-evoked fear. We found WAY significantly inhibited the anxiolytic effects of Kiss1 (P < 0.001) with an exception of freezing behaviour. Based on this, we utilised 92.79 mg/kg methysergide, a 5-HT1 and 5-HT2 receptor antagonist, and found that methysergide significantly blocked the anxiolytic effects of Kiss1 in the presence of the AS (P < 0.001). From this, we conclude that Kiss1 modulates AS-evoked fear responses mediated by the 5-HT1A and 5-HT2 receptors. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping