PUBLICATION
Molecular mechanism on cadmium-induced activity changes of catalase and superoxide dismutase
- Authors
- Wang, J., Zhang, H., Zhang, T., Zhang, R., Liu, R., Chen, Y.
- ID
- ZDB-PUB-150325-15
- Date
- 2015
- Source
- International journal of biological macromolecules 77: 59-67 (Journal)
- Registered Authors
- Wang, Jing
- Keywords
- Cadmium, Catalase and superoxide dismutase, Oxidative stress
- MeSH Terms
-
- Animals
- Cadmium/metabolism
- Cadmium/toxicity*
- Catalase/chemistry
- Catalase/metabolism*
- Catalytic Domain
- Environmental Pollutants/metabolism
- Environmental Pollutants/toxicity*
- Glutathione Disulfide/metabolism
- Malondialdehyde/metabolism
- Models, Molecular
- Oxidative Stress/drug effects
- Protein Folding/drug effects
- Superoxide Dismutase/chemistry
- Superoxide Dismutase/metabolism*
- Zebrafish/metabolism
- PubMed
- 25795390 Full text @ Int. J. Biol. Macromol.
Citation
Wang, J., Zhang, H., Zhang, T., Zhang, R., Liu, R., Chen, Y. (2015) Molecular mechanism on cadmium-induced activity changes of catalase and superoxide dismutase. International journal of biological macromolecules. 77:59-67.
Abstract
Cadmium contributes to adverse effects of organisms probably because of its ability to induce oxidative stress via alterations in activities of antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), but their molecular mechanisms remain unclear. We investigated the molecular mechanism of CAT and SOD response under Cd-induced oxidative stress in the liver of zebrafish. The enzyme activity changes observed in vitro were consistent with those seen in vivo, indicating the direct interaction of CAT and SOD with Cd contributes to their activity change in vivo. Further experiments utilizing multiple spectroscopic methods, isothermal titration calorimetry and a molecular docking study were performed to explore the mechanism of molecular interaction of CAT and SOD with Cd. Different interaction patterns were found that resulted in misfolding and changed the enzyme activities. Taken together, we suggest the misfolding of CAT and SOD contributes to their activity change under Cd-induced oxidative stress in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping