ZFIN ID: ZDB-PUB-150311-9
BDNF-TrkB Axis Regulates Migration of the Lateral Line Primordium and Modulates the Maintenance of Mechanoreceptor Progenitors
Gasanov, E.V., Rafieva, L.M., Korzh, V.P.
Date: 2015
Source: PLoS One   10: e0119711 (Journal)
Registered Authors: Korzh, Vladimir
Keywords: none
MeSH Terms:
  • Animals
  • Brain-Derived Neurotrophic Factor/physiology*
  • Cell Movement
  • Embryonic Stem Cells/physiology*
  • Fish Proteins/physiology*
  • Gene Expression
  • Lateral Line System/cytology
  • Lateral Line System/embryology*
  • Mechanoreceptors/physiology*
  • Receptor, trkB/metabolism*
  • Zebrafish/embryology*
PubMed: 25751404 Full text @ PLoS One
BDNF and its specialized receptor TrkB are expressed in the developing lateral line system of zebrafish, but their role in this organ is unknown. To tackle this problem in vivo, we used transgenic animals expressing fluorescent markers in different cell types of the lateral line and combined a BDNF gain-of-function approach by BDNF mRNA overexpression and by soaking embryos in a solution of BDNF, with a loss-of-function approach by injecting the antisence ntrk2b-morpholino and treating embryos with the specific Trk inhibitor K252a. Subsequent analysis demonstrated that the BDNF-TrkB axis regulates migration of the lateral line primordium. In particular, BDNF-TrkB influences the expression level of components of chemokine signaling including Cxcr4b, and the generation of progenitors of mechanoreceptors, at the level of expression of Atoh1a-Atp2b1a.