PUBLICATION
Mortality Caused by Bath Exposure of Zebrafish (Danio rerio) Larvae to Nervous Necrosis Virus Is Limited to the Fourth Day Postfertilization
- Authors
- Morick, D., Faigenbaum, O., Smirnov, M., Fellig, Y., Inbal, A., Kotler, M.
- ID
- ZDB-PUB-150311-18
- Date
- 2015
- Source
- Applied and environmental microbiology 81(10): 3280-7 (Journal)
- Registered Authors
- Inbal, Adi
- Keywords
- none
- MeSH Terms
-
- Larva/growth & development*
- Larva/virology
- RNA Virus Infections/mortality
- RNA Virus Infections/physiopathology
- RNA Virus Infections/veterinary*
- RNA Virus Infections/virology
- Female
- Fish Diseases/mortality*
- Fish Diseases/physiopathology
- Fish Diseases/virology
- Molecular Sequence Data
- Nodaviridae/physiology*
- Animals
- Fertilization
- Male
- Zebrafish/growth & development
- Zebrafish/physiology
- Zebrafish/virology*
- PubMed
- 25746990 Full text @ Appl. Environ. Microbiol.
Citation
Morick, D., Faigenbaum, O., Smirnov, M., Fellig, Y., Inbal, A., Kotler, M. (2015) Mortality Caused by Bath Exposure of Zebrafish (Danio rerio) Larvae to Nervous Necrosis Virus Is Limited to the Fourth Day Postfertilization. Applied and environmental microbiology. 81(10):3280-7.
Abstract
Nervous necrosis virus (NNV) is a member of the Betanodavirus family that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV by bath-immersion at 2, 4, 6 and 8 days post-fertilization (dpf). Here, we demonstrate that developing zebrafish embryos are resistant to NNV at 2 dpf due to protection afforded by the egg chorion and, to a lesser extent, by the perivitellin fluid. The zebrafish larvae succumbed to NNV infection during a narrow time "window" around the 4(th) dpf, while 6- and 8-day-old larvae were much less sensitive, with mortalities of 24% and 28%, respectively.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping