PUBLICATION
Mechanical Vessel Injury in Zebrafish Embryos
- Authors
- Clay, H., Coughlin, S.R.
- ID
- ZDB-PUB-150306-4
- Date
- 2015
- Source
- Journal of visualized experiments : JoVE (96): e52460 (Journal)
- Registered Authors
- Clay, Hilary
- Keywords
- none
- MeSH Terms
-
- Animals
- Blood Vessels/injuries*
- Disease Models, Animal*
- Hemostasis
- Microscopy, Video/methods
- Time-Lapse Imaging/methods
- Wound Healing
- Zebrafish/embryology*
- PubMed
- 25742284 Full text @ J. Vis. Exp.
Citation
Clay, H., Coughlin, S.R. (2015) Mechanical Vessel Injury in Zebrafish Embryos. Journal of visualized experiments : JoVE. (96):e52460.
Abstract
Zebrafish (Danio rerio) embryos have proven to be a powerful model for studying a variety of developmental and disease processes. External development and optical transparency make these embryos especially amenable to microscopy, and numerous transgenic lines that label specific cell types with fluorescent proteins are available, making the zebrafish embryo an ideal system for visualizing the interaction of vascular, hematopoietic, and other cell types during injury and repair in vivo. Forward and reverse genetics in zebrafish are well developed, and pharmacological manipulation is possible. We describe a mechanical vascular injury model using micromanipulation techniques that exploits several of these features to study responses to vascular injury including hemostasis and blood vessel repair. Using a combination of video and timelapse microscopy, we demonstrate that this method of vascular injury results in measurable and reproducible responses during hemostasis and wound repair. This method provides a system for studying vascular injury and repair in detail in a whole animal model.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping