PUBLICATION
Anion Exchanger 1b in Stereocilia Is Required for the Functioning of Mechanotransducer Channels in Lateral-Line Hair Cells of Zebrafish
- Authors
- Lin, Y.H., Hung, G.Y., Wu, L.C., Chen, S.W., Lin, L.Y., Horng, J.L.
- ID
- ZDB-PUB-150214-2
- Date
- 2015
- Source
- PLoS One 10: e0117041 (Journal)
- Registered Authors
- Horng, Jiun-Lin
- Keywords
- Zebrafish, Larvae, Microelectrodes, Morpholino, Cytoskeleton, Immunohistochemistry techniques, In situ hybridization, Anions
- MeSH Terms
-
- Animals
- Anion Exchange Protein 1, Erythrocyte/genetics
- Anion Exchange Protein 1, Erythrocyte/metabolism*
- Calcium/metabolism
- Gene Knockdown Techniques
- Mechanoreceptors/metabolism*
- Mechanotransduction, Cellular*
- Protein Transport
- Stereocilia/metabolism*
- Zebrafish/physiology*
- PubMed
- 25679789 Full text @ PLoS One
Citation
Lin, Y.H., Hung, G.Y., Wu, L.C., Chen, S.W., Lin, L.Y., Horng, J.L. (2015) Anion Exchanger 1b in Stereocilia Is Required for the Functioning of Mechanotransducer Channels in Lateral-Line Hair Cells of Zebrafish. PLoS One. 10:e0117041.
Abstract
The anion exchanger (AE) plays critical roles in physiological processes including CO2 transport and volume regulation in erythrocytes and acid-base regulation in renal tubules. Although expression of the AE in inner-ear hair cells was reported, its specific localization and function are still unclear. Using in situ hybridization, we found that the AE1b transcript is expressed in lateral-line hair cells of zebrafish larvae. An immunohistochemical analysis with a zebrafish-specific antibody localized AE1b to stereocilia of hair cells, and the expression was eliminated by morpholino knockdown of AE1b. A non-invasive, scanning ion-selective electrode technique was applied to analyze mechanotransducer (MET) channel-mediated Ca2+ influx at stereocilia of hair cells of intact fish. Ca2+ influx was effectively suppressed by AE1b morpholino knockdown and inhibitor (DIDS) treatment. Elevating external Ca2+ (0.2 to 2 mM) neutralized the inhibition of DIDS. Taken together, this study provides solid evidence to show that AE1b in stereocilia is required for the proper functioning of MET channels.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping